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Selection of viable spermatozoa from testicular biopsies: a comparative study between pentoxifylline and hypoosmotic
Vijay Mangoli1, Ranjana Mangoli, Sucheta Dandekar
1Fertility Clinic and IVF Center, Mumbai, India. ivflab@mtnl.net.in
Objective:
To compare fertilization, cleavage, and pregnancy outcome using pentoxifylline and a hypoosmotic swelling (HOS) test to select viable spermatozoa from testicular biopsy specimens.
Design:
Open, comparative, prospective study.
Setting:
G.S. Medical College and Fertility clinic, Mumbai, India.
Patient(S):
A total of 50 couples enrolled for infertility treatment having a male factor indication of nonobstructive azoospermia.
Intervention(S):
Assessment of viable spermatozoa using pentoxifylline and using an HOS test from a population of nonmotile spermatozoa obtained from testicular biopsies.
Main Outcome Measure(S):
Comparison of fertilization, cleavage, and clinical pregnancy rates using viable sperms recovered using pentoxifylline and an HOS test.
Result(S):
Viable spermatozoa were obtained in both the study groups. Significantly higher fertilization rates (pentoxifylline 62.05% vs. HOS 41.07%) and clinical pregnancy rates (pentoxifylline 32% vs. HOS 16%) were observed. There was no significant difference in cleavage rates among both groups.
Conclusion(S):
We found that obtaining viable spermatozoa using pentoxifylline was more effective in terms of fertilization and pregnancies than obtaining it through an HOS test.
