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Rapid Antimicrobial Susceptibility Testing by Stimulated Raman Scattering Imaging of Deuterium Incorporation in a Single Bacterium
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A biosensor platform for rapid antimicrobial susceptibility testing directly from clinical samples.

Kathleen E Mach1, Ruchika Mohan, Ellen Jo Baron

  • 1Department of Urology, Stanford University School of Medicine, Stanford, California, USA.

The Journal of Urology
|November 16, 2010
PubMed
Summary

This study introduces a novel biosensor-based antimicrobial susceptibility test for urinary tract infections. The rapid test provides accurate results within 3.5 hours, aiding in efficient antibiotic management and combating drug resistance.

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Area of Science:

  • Microbiology
  • Biosensor Technology
  • Infectious Diseases

Background:

  • Standard antibiotic susceptibility testing lacks point-of-care results, leading to empirical antibiotic use.
  • Empirical antibiotic use contributes to the rise of drug-resistant pathogens.
  • Rapid diagnostics are crucial for effective antibiotic stewardship.

Purpose of the Study:

  • To develop a point-of-care biosensor for determining antibiotic susceptibility of urinary tract pathogens.
  • To create a rapid antimicrobial susceptibility test (AST) for direct use with patient samples.

Main Methods:

  • Assay development involved culturing bacteria with antibiotics and measuring growth via electrochemical detection of bacterial 16S rRNA.
  • Patient urine samples were cultured on antibiotic plates for 2.5 hours, with growth quantified by biosensor.
  • Validation involved comparing biosensor AST results with standard microbiological analysis for 252 urine samples.

Main Results:

  • Electrochemical quantitation of bacterial 16S rRNA effectively monitors bacterial growth for AST.
  • The biosensor-based AST demonstrated 94% accuracy across 368 pathogen-antibiotic tests compared to standard methods.
  • The test was successfully validated using urine samples from patients with urinary tract infections.

Conclusions:

  • The biosensor-based antimicrobial susceptibility test provides rapid culture and susceptibility information from urine samples.
  • This test can deliver results within 3.5 hours, significantly reducing diagnostic delays.
  • Combined with a pathogen identification assay, this biosensor offers a complete point-of-care solution for urinary tract infections.