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Use of Galleria mellonella as a Model Organism to Study Legionella pneumophila Infection
Published on: November 22, 2013
[Legionellosis in a site with low risk of proliferation]
Helena Pañella1, Núria Calzada, Vicente Beneyto
1Agència de Salut Pública de Barcelona, Direcció de Serveis de Vigilància Ambiental, Barcelona, España. hpanella@aspb.cat
Abstract:
After three cases of legionellosis associated with a spa were identified, an investigation was conducted to confirm the source of infection, determine the risk factors, and establish control measures. Between November 26, 2008 and September 16, 2009, six inspections were carried out, samples were collected for Legionella pneumophila isolation, and water temperature was determined at all the sampling points of the hot water system and at the spa pools. The presence of L. pneumophila serogroup 1 with the same molecular pattern as that found in the clinical isolate was confirmed in the hot water system (with a low proliferation risk according to legislation). The crude attack rate was 0.34% (95% CI: 0.09-0.94). Samples without L. pneumophila were obtained only after structural changes were carried out. Possible factors participating in the cluster were water temperatures between 25 °C and 30 °C, stagnant water and early morning exposure.
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