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Updated: Jun 6, 2026

Preparation, Purification, and Use of Fatty Acid-containing Liposomes
Published on: February 9, 2018
On the surface properties of oleate micelles and oleic acid/oleate vesicles studied by spin labeling
Branka Dejanović1, Vesna Noethig-Laslo, Marjeta Sentjurc
1Department of Physical Chemistry, "Ruđer Bošković" Institute, 10002 Zagreb, Croatia.
Abstract:
Dilute aqueous systems composed of sodium oleate micelles and sodium oleate/oleic acid vesicles were investigated as a function of pH by electron spin resonance spectroscopy with TEMPO-stearate TEMPO-stearamide as well as with a positively charged water soluble spin label, TEMPO-choline. The dynamics of the three TEMPO-spin labels were found to be sensitive to changes in the interfacial region of the aggregates as a function of pH. The results obtained are consistent with the formation of a hydrogen bond network (RCOO(-)↔HOOCR) at the surface of the sodium oleate/oleic acid system in the course of the transformation of micelles into the closed bilayers (vesicles). Vesicles formation below pH 10 was determined independently with a spin labeled glucose derivative.
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