Related Experiment Videos
Heat-inducible translational coupling in Bacillus subtilis.
S Fujiwara1, N Tsubokura, Y Kurusu
1Department of Applied Biochemistry, Faculty of Applied Biological Sciences, Hiroshima University, Japan.
Nucleic Acids Research
|February 25, 1990
Summary
Bacillus subtilis plasmid pGR71-369 exhibits heat-inducible cat gene expression. This heat inducibility is mediated by translational coupling within the upstream regulatory region, offering insights into bacterial gene regulation.
Area of Science:
- Microbiology
- Molecular Biology
- Genetics
Background:
- Bacillus subtilis plasmid pGR71 is a promoter-probe shuttle vector.
- Expression of the cat gene requires a Bacillus promoter and ribosomal binding site (RBS).
Purpose of the Study:
- To investigate the mechanism of heat-inducible cat gene expression in a modified Bacillus subtilis plasmid, pGR71-369.
- To identify the regulatory region responsible for heat inducibility.
Main Methods:
- Nucleotide sequence analysis of the upstream region of the cat gene.
- Deletion analysis of the plasmid.
- Dot blot hybridization analysis of mRNA under various conditions.
Main Results:
- A spontaneous deletion in pGR71-369 resulted in heat-inducible cat gene expression in B. subtilis.
- Heat inducibility is mediated by translational coupling.
- The regulatory region for heat inducibility is located upstream of the cat gene.
Conclusions:
- The cat gene expression in pGR71-369 is regulated by heat-inducible translational coupling.
- The upstream region of the cat gene contains the necessary regulatory elements for heat inducibility.