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A Murine Model of Dengue Virus-induced Acute Viral Encephalitis-like Disease

A Murine Model of Dengue Virus-induced Acute Viral Encephalitis-like Disease

Published on: April 28, 2019

Mouse STAT2 restricts early dengue virus replication.

Joseph Ashour¹, Juliet Morrison, Maudry Laurent-Rolle

¹Department of Microbiology, Mount Sinai School of Medicine, New York, NY 10029, USA.

Cell Host & Microbe

|November 16, 2010

Summary

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Dengue virus NS5 protein degrades human STAT2 but not mouse STAT2, crucial for viral replication. This species-specific interaction highlights STAT2

Area of Science:

Virology
Immunology
Molecular Biology

Background:

Dengue virus employs interferon antagonists to evade host immunity.
The NS5 protein targets STAT2, a key component of the type I interferon signaling pathway, for degradation.

Purpose of the Study:

To investigate the species specificity of dengue NS5 protein's interaction with STAT2.
To elucidate the role of NS5-mediated STAT2 degradation in dengue virus replication.
To identify the region within STAT2 responsible for species-specific binding and degradation by NS5.

Main Methods:

Comparative analysis of dengue NS5 protein's interaction with human and mouse STAT2.
Assessment of dengue virus replication in the presence and absence of functional STAT2.
Mapping of STAT2 regions involved in NS5 binding and degradation.

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Isolation and Quantification of Zika Virus from Multiple Organs in a Mouse

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A Murine Model of Dengue Virus-induced Acute Viral Encephalitis-like Disease

A Murine Model of Dengue Virus-induced Acute Viral Encephalitis-like Disease

Published on: April 28, 2019

Measuring Dengue Virus RNA in the Culture Supernatant of Infected Cells by Real-time Quantitative Polymerase Chain Reaction

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Isolation and Quantification of Zika Virus from Multiple Organs in a Mouse

Isolation and Quantification of Zika Virus from Multiple Organs in a Mouse

Published on: August 15, 2019

Main Results:

Dengue NS5 protein specifically binds and degrades human STAT2, but not mouse STAT2.
NS5-mediated STAT2 antagonism is essential for efficient dengue virus replication.
The coiled-coil domain of STAT2 mediates species-specific interactions with dengue NS5.
Mouse STAT2 restricts early dengue virus replication in vivo.

Conclusions:

The species specificity of STAT2 interaction with dengue NS5 is a critical factor in viral replication.
Targeting this species-specific interaction could be a strategy for antiviral development.
Developing transgenic mouse models expressing human STAT2 may facilitate dengue research.