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Updated: Jun 6, 2026

Investigation of Protein Recruitment to DNA Lesions Using 405 Nm Laser Micro-irradiation
Published on: March 20, 2018
Study of DNA damage with a new system for irradiation of samples in a nuclear reactor
Maritza R Gual1, Felix M Milian, Airton Deppman
1Instituto Superior de Tecnologías y Ciencias Aplicadas, InSTEC; Avenida Salvador Allende y Luaces, Quinta de Los Molinos, Plaza de la Revolución, Havana, AP 6163, Cuba. mrgual@instec.cu
Abstract:
In this paper, we report results of a quantitative analysis of the effects of neutrons on DNA, and, specifically, the production of simple and double breaks of plasmid DNA in aqueous solutions with different concentrations of free-radical scavengers. The radiation damage to DNA was evaluated by electrophoresis through agarose gels. The neutron and gamma doses were measured separately with thermoluminescent detectors. In this work, we have also demonstrated usefulness of a new system for positioning and removing samples in channel BH#3 of the IEA-R1 reactor at the Instituto de Pesquisas Energéticas e Nucleares (Brazil) without necessity of interrupting the reactor operation.
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