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Related Concept Videos

Mesenchymal Stem Cells01:19

Mesenchymal Stem Cells

Mesenchymal stem cells (MSCs) are adult stem cells that can differentiate into most connective tissue cell types, except for hematopoietic cells, depending upon the source of MSCs. For example, bone-marrow-derived MSCs (BM-MSCs) can differentiate into osteocytes, hepatocytes, and pancreatic and neuronal cells. MSCs can be isolated from various sources such as bone marrow, placenta, adipose tissue, teeth, and Wharton’s jelly, a gelatinous substance in the umbilical cord. The ease of their access...

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Technique for Isolation and Culture of Rat Jaw Bone Marrow Mesenchymal Stem Cells
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Technique for Isolation and Culture of Rat Jaw Bone Marrow Mesenchymal Stem Cells

Published on: May 31, 2024

Mouse mandible contains distinctive mesenchymal stem cells.

T Yamaza1, G Ren, K Akiyama

  • 1Center for Craniofacial Molecular Biology, University of Southern California School of Dentistry, 2250 Alcazar Street, CSA 103, Los Angeles, CA 90033, USA.

Journal of Dental Research
|November 16, 2010
PubMed
Summary

Researchers successfully isolated mouse orofacial mesenchymal stem cells (OMSCs), distinct from bone marrow mesenchymal stem cells (BMMSCs). These OMSCs play a unique role in regulating T-lymphocytes and systemic immunity.

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Isolation and Cultivation of Mandibular Bone Marrow Mesenchymal Stem Cells in Rats
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Isolation and Cultivation of Mandibular Bone Marrow Mesenchymal Stem Cells in Rats

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Isolation and Cultivation of Mandibular Bone Marrow Mesenchymal Stem Cells in Rats
08:58

Isolation and Cultivation of Mandibular Bone Marrow Mesenchymal Stem Cells in Rats

Published on: August 25, 2020

Area of Science:

  • Stem cell biology
  • Immunology
  • Craniofacial research

Background:

  • Mesenchymal stem cells (MSCs) from human orofacial bone show distinct differentiation compared to bone marrow MSCs (BMMSCs).
  • Technical challenges have hindered the isolation and study of mouse orofacial bone-derived MSCs (OMSCs).
  • This limitation impacts the development of mouse models for orofacial diseases.

Purpose of the Study:

  • To develop a method for isolating and expanding mouse OMSCs.
  • To characterize the properties of OMSCs.
  • To investigate the interaction between OMSCs and T-lymphocytes.

Main Methods:

  • Isolation and expansion of OMSCs from mouse mandibles.
  • Verification of MSC characteristics: single-colony formation, multi-lineage differentiation, and in vivo tissue regeneration.
  • Assessment of OMSC interaction with activated T-lymphocytes via the Fas/Fas ligand pathway.

Main Results:

  • Successfully isolated and expanded OMSCs with verified MSC characteristics.
  • OMSCs demonstrated distinct properties compared to BMMSCs in regulating T-lymphocyte survival and proliferation.
  • Activated T-lymphocytes impaired OMSCs through the Fas/Fas ligand pathway.

Conclusions:

  • OMSCs represent a unique MSC population.
  • OMSCs possess distinct immunomodulatory functions compared to BMMSCs.
  • OMSCs play a significant role in systemic immunity and offer potential for orofacial disease research.