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Related Concept Videos

Bioreactor Design and Operational System01:29

Bioreactor Design and Operational System

Bioreactors are engineered vessels designed to cultivate microorganisms under controlled conditions for industrial bioprocessing. They maintain sterility and allow precise regulation of pH, temperature, oxygen, and nutrient levels to optimize microbial growth and metabolite production. Bioreactors range from small laboratory units of 1 liter to industrial systems holding up to 500,000 liters, though only about 75% of their volume is actively used for fermentation. The remaining headspace...
Bioreactor Controls-II01:18

Bioreactor Controls-II

In aerobic fermentations, oxygen is vital for microbial growth and metabolite production. Since air comprises only about 20% oxygen and the gas is poorly soluble in water—just 9 ppm at 20°C—supplying sufficient oxygen becomes a critical challenge, especially in high-demand processes like yeast growth or citric acid production. Even a fully saturated broth may offer only a few seconds of oxygen availability.To address this, sterile or scrubbed air is introduced into the fermentor via a sparger...
Scale-Up Processes01:14

Scale-Up Processes

The scale-up of microbial fermentation processes is essential in industrial biotechnology, allowing the transition from laboratory-scale experiments to commercial-scale production while aiming to maintain product yield and quality. This process requires meticulous adjustment of equipment design, process parameters, and contamination control strategies to accommodate increasing culture volumes.At the laboratory scale, cultures are typically maintained in 1 to 10-liter glass or autoclavable...
Designing Growth Media for Bioreactors01:30

Designing Growth Media for Bioreactors

Growth media provide essential nutrients that support cell growth and metabolism, thereby enhancing the yield of valuable products such as enzymes, antibiotics, and biomass. Designing an effective growth medium involves balancing all components to prevent nutrient limitations or toxic excesses, both of which can impair growth and reduce product yields.Composition of a Typical Growth MediumA typical growth medium contains carbon and nitrogen sources, salts, vitamins, trace elements, and...
Bioreactor Controls-I01:28

Bioreactor Controls-I

Maintaining optimal conditions within fermenters is essential for maximizing microbial productivity and ensuring process efficiency. This lesson focuses on key parameters—temperature, foam, pH, carbon dioxide, oxygen, and pressure—and their precise measurement and control strategies in fermentation systems.Temperature ControlTemperature regulation is critical due to the exothermic nature of many fermentation processes. In small laboratory fermenters, temperature is commonly monitored using...
Typical Model Studies01:30

Typical Model Studies

Fluid mechanics model studies often utilize scaled-down systems to predict fluid behavior in full-scale environments, such as river flows, dam spillways, and structures interacting with open surfaces. Maintaining Froude number similarity in river models is crucial, as it replicates surface flow features like wave patterns and velocities.

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Related Experiment Video

Updated: Jun 6, 2026

A Novel Bioreactor for High Density Cultivation of Diverse Microbial Communities
08:13

A Novel Bioreactor for High Density Cultivation of Diverse Microbial Communities

Published on: December 25, 2015

Modelling a full scale membrane bioreactor using Activated Sludge Model No.1: challenges and solutions.

F Delrue1, J M Choubert, A E Stricker

  • 1Cemagref, UR QELY, F-69336 Lyon Cedex 09, France. florian.delrue@cemagref.fr

Water Science and Technology : a Journal of the International Association on Water Pollution Research
|November 16, 2010
PubMed
Summary
This summary is machine-generated.

This study calibrated the Activated Sludge Model No. 1 (ASM1) for a full-scale membrane bioreactor (MBR). The model accurately predicted mixed liquor suspended solids despite challenges in wastewater characterization and oxygen transfer calibration.

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Area of Science:

  • Environmental Engineering
  • Biotechnology
  • Wastewater Treatment

Background:

  • Membrane bioreactors (MBRs) are advanced wastewater treatment systems.
  • Accurate modeling is crucial for optimizing MBR performance.
  • The Activated Sludge Model No. 1 (ASM1) is a common tool for simulating biological wastewater treatment.

Purpose of the Study:

  • To calibrate the ASM1 model for a full-scale MBR.
  • To investigate the impact of complex aeration systems on model calibration.
  • To assess the model's predictive capability for sludge production and quality.

Main Methods:

  • Wastewater characterization and fractionation.
  • Monitoring of mixed liquor volatile suspended solids (MLVSS) and mixed liquor suspended solids (MLSS).
  • Calibration and validation of ASM1 parameters, including biomass kinetics and oxygen transfer.

Main Results:

  • Wastewater fractionation required adjustment based on sludge production rates.
  • MLVSS and MLSS were accurately predicted during calibration and validation.
  • Simultaneous nitrification and denitrification due to complex aeration complicated oxygen transfer calibration.

Conclusions:

  • A calibrated ASM1 model provided satisfactory results for the MBR.
  • Model performance decreased during validation due to changing aeration configurations.
  • This study represents the first simulation of a full-scale MBR plant using ASM1.