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High molecular weight aggregate content of heated and unheated factor VIII products determined by fast-protein liquid
J Dawes1, L Freeman, N J Dawson
1Heart Research Institute, Sydney, Australia.
Vox Sanguinis
|January 1, 1990
Abstract:
The molecular-weight distribution of proteins in factor VIII concentrates has been analysed by fast-protein liquid chromatography. The proportion of high-molecular-weight (HMW) aggregates in one product increased on freeze-drying and heating, with fibrinogen and fibronectin being the main protein components of the HMW peak. In all other concentrates, the HMW peak was less than or equal to 5% of the total protein content and there were no differences in HMW content according to purity or method of viral inactivation.