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Immunogold Electron Microscopy

Immunogold Electron Microscopy

Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.

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Related Experiment Video

Updated: Jun 6, 2026

Rapid Nanoprobe Signal Enhancement by In Situ Gold Nanoparticle Synthesis

Rapid Nanoprobe Signal Enhancement by In Situ Gold Nanoparticle Synthesis

Published on: March 7, 2018

Direct Interaction Between Gold Nanorods and Glucose.

Xiangling Ren, Liuqing Yang, Jun Ren

Nanoscale Research Letters

|November 16, 2010

Summary

Gold nanorods (GNRs) show sensitive optical property changes with glucose concentration. Glucose significantly alters GNRs optical properties, suggesting potential for glucose sensing applications.

Area of Science:

Nanotechnology
Biomedical Optics
Materials Science

Background:

Gold nanorods (GNRs) possess unique optical properties.
Glucose detection is crucial in various biomedical applications.
Understanding nanoparticle-analyte interactions is key for sensor development.

Purpose of the Study:

To investigate the interaction between gold nanorods and glucose.
To evaluate the sensitivity of GNR optical properties to glucose concentration.
To propose a mechanism for GNR-glucose interaction.

Main Methods:

Synthesis and characterization of gold nanorods.
Spectroscopic analysis of GNRs in the presence of varying glucose concentrations.
Comparative analysis with gold nanospheres.

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Last Updated: Jun 6, 2026

Rapid Nanoprobe Signal Enhancement by In Situ Gold Nanoparticle Synthesis

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Published on: March 7, 2018

Gold Nanorod-assisted Optical Stimulation of Neuronal Cells

Gold Nanorod-assisted Optical Stimulation of Neuronal Cells

Published on: April 27, 2015

Synthesis of Near-Infrared Emitting Gold Nanoclusters for Biological Applications

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Main Results:

Gold nanorods exhibit higher sensitivity to glucose than gold nanospheres.
Increasing glucose concentration leads to a decrease in the long-wavelength bands of GNRs.
High glucose concentrations cause the absorption peak to disappear and reduce transverse plasmon band intensities.

Conclusions:

Glucose significantly affects the optical properties of gold nanorods.
A potential interaction mechanism between GNRs and glucose has been proposed.
The findings suggest GNRs as promising candidates for glucose sensing applications.