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Related Concept Videos

Microbes in Beverage Production01:25

Microbes in Beverage Production

Alcoholic beverages such as wine, beer, and spirits are the products of microbial fermentation processes that transform simple sugars into ethanol and a wide array of complex flavor compounds. These transformations rely on the metabolic activities of specific yeasts and bacteria, which are selected and controlled to yield the desired beverage characteristics.Wine Fermentation and MaturationWine production begins with the crushing of grapes to release juice and pulp, forming a must that is...
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Strain improvement is a foundational strategy in industrial microbiology aimed at maximizing microbial productivity, particularly because natural isolates typically yield commercially valuable products in very low concentrations. Although optimizing the culture medium and environmental conditions can improve yields, these adjustments are inherently limited by the organism’s genetic potential. As a result, the focus shifts toward genetic modifications to enhance biosynthetic capacity. The...
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Profiling the Bacterial Community of Fermenting Traminette Grapes during Wine Production using Metagenomic Amplicon Sequencing
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Microvinification--how small can we go?

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Summary

Microtiter plates (MTPs) are suitable for screening microorganisms, offering similar yeast growth to anaerobic flasks. However, ethanol evaporation in MTPs affects metabolic profiles, limiting their use for certain analyses.

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Area of Science:

  • Microbiology
  • Biotechnology
  • Fermentation Science

Background:

  • High-throughput screening of microorganisms requires small-scale culture vessels.
  • Microtiter plates (MTPs) are increasingly adopted for phenotypic screening of aerobic cultures.
  • Previous assessments focused on MTP suitability for various applications.

Purpose of the Study:

  • To evaluate the metabolic footprint of MTP fermentation.
  • To compare MTP fermentation with traditional flask methods (anaerobic and aerobic).
  • To determine the utility of MTPs for industrial strain and media screening.

Main Methods:

  • Chardonnay grape juice fermentation was conducted in MTPs.
  • Comparisons were made with fermentations in air-locked (anaerobic) and cotton-plugged (aerobic) flasks.
  • Yeast growth rates, biomass accumulation, and key metabolite profiles were analyzed.

Main Results:

  • Yeast growth rates and biomass in MTPs were comparable to anaerobic flask cultures.
  • Glycerol and acetate accumulation in MTPs mirrored anaerobic cultures.
  • Ethanol accumulation was reduced in MTPs due to increased evaporation in the small-scale format.

Conclusions:

  • Microtiter plates can serve as a viable alternative to self-induced anaerobic flasks for specific applications.
  • MTPs offer a cost-effective platform for screening industrial yeast strains and culture media.
  • Careful consideration of metabolite volatility, like ethanol, is necessary when using MTPs.