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Related Concept Videos

Capillary Electrophoresis: Applications01:30

Capillary Electrophoresis: Applications

Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...

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Membrane Transport Processes Analyzed by a Highly Parallel Nanopore Chip System at Single Protein Resolution
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Published on: August 16, 2016

Microchip electroseparation of proteins using lipid-based nanoparticles.

Pelle Ohlsson1, Olga Ordeig, Christian Nilsson

  • 1Department of Micro and Nanotechnology, Technical University of Denmark, Kongens Lyngby, Denmark.

Electrophoresis
|November 16, 2010
PubMed
Summary

Porous liquid crystalline lipid-based nanoparticles significantly reduce protein adsorption in microchip electroseparation, enhancing separation quality and reproducibility. This advance improves the analysis of complex protein samples, including challenging separations of green fluorescent protein mutants.

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Last Updated: Jun 6, 2026

Membrane Transport Processes Analyzed by a Highly Parallel Nanopore Chip System at Single Protein Resolution
11:55

Membrane Transport Processes Analyzed by a Highly Parallel Nanopore Chip System at Single Protein Resolution

Published on: August 16, 2016

Digital Microfluidics for Automated Proteomic Processing
10:55

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Published on: November 6, 2009

Electrophoretic Separation of Proteins
08:17

Electrophoretic Separation of Proteins

Published on: June 12, 2008

Area of Science:

  • Analytical Chemistry
  • Nanotechnology
  • Biochemistry

Background:

  • Protein adsorption to channel walls in microchip electroseparation hinders analysis, leading to peak broadening and reduced sensitivity.
  • Effective strategies are needed to minimize sample adsorption for improved separation performance.

Purpose of the Study:

  • To investigate the use of porous liquid crystalline lipid-based nanoparticles to reduce protein adsorption in microchip electroseparation.
  • To evaluate the impact of these nanoparticles on separation stability, reproducibility, and resolution.

Main Methods:

  • Green fluorescent protein (GFP) and its mutants were analyzed using hot embossed cyclic olefin polymer microchips with integrated fiber grooves for laser-induced fluorescence (LIF) detection.
  • Porous liquid crystalline lipid-based nanoparticles were added to the sample and background electrolyte at varying concentrations (0.5-10%).
  • Sample adsorption was indirectly quantified by analyzing peak height, width, and asymmetry in electropherograms.

Main Results:

  • Addition of nanoparticles dramatically reduced protein adsorption, evidenced by higher, narrower, and more symmetric separation peaks.
  • Nanoparticle addition decreased channel current and enabled separations of GFP mutants that were previously hindered by adsorption.
  • Increased nanoparticle concentration improved the resolution of impurities in GFP samples, suggesting reduced adsorption and/or a pseudostationary phase effect.

Conclusions:

  • Porous liquid crystalline lipid-based nanoparticles are effective in mitigating protein adsorption during microchip electroseparation.
  • The use of these nanoparticles enhances separation performance, stability, and the ability to resolve complex protein mixtures.
  • This approach offers a promising strategy for improving protein analysis in microfluidic devices.