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Related Concept Videos

Adult Stem Cells01:33

Adult Stem Cells

Stem cells are undifferentiated cells that divide and produce more stem cells or progenitor cells that differentiate into mature, specialized cell types. All the cells in the body are generated from stem cells in the early embryo, but small populations of stem cells are also present in many adult tissues including the bone marrow, brain, skin, and gut. These adult stem cells typically produce the various cell types found in that tissue—to replace cells that are damaged or to continuously renew...
Embryonic Stem Cells00:58

Embryonic Stem Cells

Embryonic stem (ES) cells are undifferentiated pluripotent cells, meaning they can produce any cell type in the body. This gives them tremendous potential in science and medicine since they can generate specific cell types for use in research or to replace body cells lost due to damage or disease.
Embryonic Stem Cells00:57

Embryonic Stem Cells

Embryonic stem (ES) cells were first discovered in mice in 1981 by Martin Evans. In 1998, James Thomson identified a method to isolate embryonic stem cells from humans. Human embryonic stem cells (hESCs) are obtained from 3-5 day old embryos that remain unused after an in vitro fertilization procedure.
ES cells are grown in a culture medium where they can divide indefinitely, creating ES cell lines. Under certain conditions, ES cells can differentiate, either spontaneously into a variety of...
Stem Cell Culture01:17

Stem Cell Culture

Stem cell research aims to find ways to use stem cells to regenerate and repair cellular damage. Over time, most adult cells undergo the wear and tear of aging and lose their ability to divide and repair themselves. Stem cells do not display a particular morphology or function. Adult stem cells, which exist as a small subset of cells in most tissues, keep dividing and can differentiate into a number of specialized cells generally formed by that tissue. These cells enable the body to renew and...
Zygotic Development And Stem Cell Formation01:10

Zygotic Development And Stem Cell Formation

The development of all multicellular organisms starts with the fusion of haploid cells called sperm and egg to form a diploid zygote. A zygote is a totipotent cell that can develop into a complete organism. The zygote undergoes cell division or cleavage to form an 8-cell mass. Until this stage, the cells are spherical, loosely attached, and remain totipotent. Totipotent cells are capable of developing both the embryonic and the extraembryonic tissues. However, as they continue to divide, they...

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Related Experiment Video

Updated: Jun 6, 2026

The Production of Pluripotent Stem Cells from Mouse Amniotic Fluid Cells Using a Transposon System
08:24

The Production of Pluripotent Stem Cells from Mouse Amniotic Fluid Cells Using a Transposon System

Published on: February 28, 2017

[Stem cells in human amniotic fluid].

D A Davydova

    Izvestiia Akademii Nauk. Seriia Biologicheskaia
    |November 17, 2010
    PubMed
    Summary

    Amniotic fluid stem cells possess mesenchymal, neural, and epithelial markers, indicating potential for diverse differentiation. These stem cells, found in amniotic fluid, offer promising therapeutic applications.

    Area of Science:

    • Stem cell biology
    • Regenerative medicine
    • Fetal cell research

    Background:

    • Amniotic fluid (AF) is a source of fetal cells, including stem cells.
    • AF stem cells exhibit characteristics of mesenchymal and neural lineages.
    • Previous research suggests AF stem cells have differentiation capabilities.

    Purpose of the Study:

    • To characterize the marker expression profile of stem cells from amniotic fluid.
    • To investigate the differentiation potential of AF stem cells.
    • To discuss the status and potential applications of AF stem cells.

    Main Methods:

    • Flow cytometry for marker analysis (CD73, CD90, CD105, Nestin, beta3-tubulin, NEFH, Oct4, Nanog).
    • Immunohistochemistry to detect epithelial markers (Keratin 19, Keratin 18, p63).

    More Related Videos

    Reprogramming Primary Amniotic Fluid and Membrane Cells to Pluripotency in Xeno-free Conditions
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    Published on: November 27, 2017

    Isolation, Cryopreservation and Culture of Human Amnion Epithelial Cells for Clinical Applications
    05:31

    Isolation, Cryopreservation and Culture of Human Amnion Epithelial Cells for Clinical Applications

    Published on: December 21, 2014

    Related Experiment Videos

    Last Updated: Jun 6, 2026

    The Production of Pluripotent Stem Cells from Mouse Amniotic Fluid Cells Using a Transposon System
    08:24

    The Production of Pluripotent Stem Cells from Mouse Amniotic Fluid Cells Using a Transposon System

    Published on: February 28, 2017

    Reprogramming Primary Amniotic Fluid and Membrane Cells to Pluripotency in Xeno-free Conditions
    09:34

    Reprogramming Primary Amniotic Fluid and Membrane Cells to Pluripotency in Xeno-free Conditions

    Published on: November 27, 2017

    Isolation, Cryopreservation and Culture of Human Amnion Epithelial Cells for Clinical Applications
    05:31

    Isolation, Cryopreservation and Culture of Human Amnion Epithelial Cells for Clinical Applications

    Published on: December 21, 2014

  • Cell culture and cloning to assess morphology and differentiation capacity.

    • Main Results:

    • AF stem cells express mesenchymal, neural, and pluripotent markers.
    • Epithelial markers (Keratin 19, Keratin 18, p63) are co-expressed with mesenchymal markers.
    • Cloning yields colonies with both fibroblast-like and epithelial-like morphologies.

    Conclusions:

    • AF stem cells represent a unique cell population with multipotent differentiation potential.
    • The co-expression of epithelial and mesenchymal markers suggests plasticity.
    • AF stem cells hold promise for regenerative medicine and therapeutic applications.