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Brain Slice Stimulation Using a Microfluidic Network and Standard Perfusion Chamber
Published on: October 1, 2007
A brain slice bath for physiology and compound microscopy, with dual-sided perifusion
1Department of Physiology, University of Otago, Dunedin, New Zealand. phil.heyward@stonebow.otago.ac.nz
Journal of Microscopy
|November 17, 2010
Summary
This study introduces a novel brain slice recording bath that optimizes tissue physiology by perfusing medium past both sides of slices. This design is compatible with standard compound microscopes for enhanced neuroscience research.
Area of Science:
- Neuroscience
- Physiological Recording
- Microscopy
Background:
- Standard in vitro brain slice studies often expose tissue to medium on only one side, potentially compromising physiology.
- Existing recording baths are not readily compatible with compound microscopes for optimal slice perfusion.
Purpose of the Study:
- To describe a novel tissue bath designed for microscopy and physiological recording.
- To enable perfusion of medium past both sides of brain slices within a compound microscope setup.
Main Methods:
- A new tissue bath design featuring concentric mesh rings to support and transport slices.
- Temperature-controlled medium flows past both sides of submerged brain slices.
- Adaptable for acute slices, cultured slices, and dispersed cells.
Main Results:
- The bath design allows optimal physiological conditions by perfusing both sides of the tissue.
- It is compatible with standard compound microscope objectives and condensers.
- The design facilitates electrode approach and is adaptable for various preparations.
Conclusions:
- The described tissue bath provides an optimal environment for brain slice physiology and imaging.
- It is robust, easy to construct, and compatible with standard microscopy equipment.
- This facilitates advanced in vitro neuroscience research.
