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Updated: Jun 6, 2026

Isolation and Flow Cytometric Analysis of Immune Cells from the Ischemic Mouse Brain
Published on: February 12, 2016
Immune responses after acute ischemic stroke or myocardial infarction
Karl Georg Haeusler1, Wolf U H Schmidt, Fabian Foehring
1Department of Neurology, Charité University Medicine Berlin, Germany. georg.haeusler@charite.de
Background:
We recently demonstrated an immediate immunodepressive state after acute ischemic stroke in humans.
Methods:
In the present study, we prospectively analyzed immune responses in patients with middle cerebral artery stroke (n=20), acute myocardial infarction (n=20) and healthy controls (n=20, also matched for age and gender).
Results:
Compared to controls, a rapid depression of monocytic HLA-DR expression and a defective lymphocytic IFN-γ production was obvious after ischemic stroke or myocardial infarction, while total counts of leukocytes and monocytes were significantly higher after myocardial infarction. A T cell-mediated lymphopenia was accentuated in patients with severe stroke, obviously predisposing these patients for nosocomial infections.
Conclusions:
Our data reveal an immediate and to some extent differential suppression of cell-mediated immune responses after ischemic stroke or myocardial infarction respectively.
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