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Expression of urokinase plasminogen activator receptor on monocytes and granulocytes is modulated by cardiac surgery

Jan Krejsek1, Martina Kolackova, Pavel Kunes

  • 1Department of Clinical Immunology and Allergology, Charles University in Prague, School of Medicine and University Hospital in Hradec Kralove, Czech Republic. ukia@fnhk.cz

Perfusion
|November 17, 2010
PubMed

Insights

Cardiac surgery significantly alters urokinase plasminogen activator receptor (uPAR) expression on monocytes and granulocytes. This modulation occurs regardless of whether standard or mini-invasive cardiopulmonary bypass is used.

Area of Science:

  • Cardiovascular Surgery
  • Immunology
  • Hematology

Background:

  • Coronary artery bypass grafting (CABG) triggers the blood coagulation/fibrinolysis cascade, a key inflammatory response post-cardiac surgery.
  • Understanding the role of urokinase plasminogen activator receptor (uPAR, CD87) in this process is crucial for managing post-surgical inflammation.

Purpose of the Study:

  • To investigate the modulation of uPAR expression on monocytes and granulocytes in patients undergoing CABG.
  • To compare uPAR expression changes between standard (
  • on-pump
  • ') and mini-invasive (
  • mini on-pump
  • ') cardiopulmonary bypass techniques.

Main Methods:

  • Flow cytometry was used to measure uPAR expression (Median Fluorescence Intensity) on blood monocytes and granulocytes.
  • uPAR expression levels were analyzed before, during, and after CABG surgery in both "on-pump" and "mini on-pump" patient groups.

Main Results:

  • In "on-pump" patients, uPAR expression on granulocytes and monocytes significantly decreased post-surgery and during the first three days.
  • In "mini on-pump" patients, uPAR expression on granulocytes and monocytes decreased significantly only after surgery.
  • No significant differences in uPAR expression modulation were observed between "on-pump" and "mini on-pump" CABG procedures.

Conclusions:

  • Cardiac surgery significantly modulates uPAR expression on both granulocytes and monocytes.
  • The observed changes in uPAR expression suggest its involvement in the inflammatory response following CABG.
  • The type of cardiopulmonary bypass (standard vs. mini-invasive) did not lead to significant differences in uPAR modulation.
Abstract

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