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Updated: Jun 6, 2026

Induction and Validation of Cellular Senescence in Primary Human Cells
Published on: June 20, 2018
The essence of senescence
Thomas Kuilman1, Chrysiis Michaloglou, Wolter J Mooi
1Division of Molecular Genetics, The Netherlands Cancer Institute, Amsterdam, The Netherlands.
Abstract:
Almost half a century after the first reports describing the limited replicative potential of primary cells in culture, there is now overwhelming evidence for the existence of "cellular senescence" in vivo. It is being recognized as a critical feature of mammalian cells to suppress tumorigenesis, acting alongside cell death programs. Here, we review the various features of cellular senescence and discuss their contribution to tumor suppression. Additionally, we highlight the power and limitations of the biomarkers currently used to identify senescent cells in vitro and in vivo.
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