Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Immunogold Electron Microscopy01:20

Immunogold Electron Microscopy

Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.
Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Diradical dications of electron-enriched sulfur-bridged dihydrophenazine derivatives.

Chemical communications (Cambridge, England)·2026
Same author

Hyperacute Rejection of a Kidney Allograft Associated with Anti-Histidyl-tRNA Synthetase and Anti-Signal Transducer and Activator of Transcription 6 Antibodies.

Kidney international reports·2026
Same author

Thickness-dependent decoupling charge transport and NH<sub>3</sub>sensing in multilayer MoS<sub>2</sub>transistors.

Nanotechnology·2026
Same author

Thermally Regulated Curing-Degradation Windows of Epoxidized Soybean Oil-Based Epoxy-Anhydride Liquid Plugs for Sustainable High-Temperature Sealing.

Molecules (Basel, Switzerland)·2026
Same author

Neutrophil extracellular trap (NET)-related index as an indicator of periprosthetic joint infection.

Journal of bone and joint infection·2026
Same author

Increased ULBP1 by doxorubicin sensitizes neuroblastoma to γδT-cell cytotoxicity.

Journal of immunology (Baltimore, Md. : 1950)·2026

Related Experiment Video

Updated: Jun 6, 2026

Synthesis of Near-Infrared Emitting Gold Nanoclusters for Biological Applications
09:11

Synthesis of Near-Infrared Emitting Gold Nanoclusters for Biological Applications

Published on: March 22, 2020

Visual detection of Hg2+ with high selectivity using thymine modified gold nanoparticles.

Xiangjun Liu1, Xiaohong Cheng, Tao Bing

  • 1Beijing National Laboratory for Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, PR China.

Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry
|November 17, 2010
PubMed
Summary

This study presents a simple colorimetric sensor for detecting mercury ions (Hg2+) in water. The sensor, using gold nanoparticles, changes color to signal mercury presence, enabling easy on-site pollution monitoring.

More Related Videos

Direct Synthesis of EM-Visible Gold Nanoparticles in Cells for Protein Localization Analysis with Well-Preserved Ultrastructure
09:22

Direct Synthesis of EM-Visible Gold Nanoparticles in Cells for Protein Localization Analysis with Well-Preserved Ultrastructure

Published on: April 28, 2023

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay
12:31

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay

Published on: February 28, 2015

Related Experiment Videos

Last Updated: Jun 6, 2026

Synthesis of Near-Infrared Emitting Gold Nanoclusters for Biological Applications
09:11

Synthesis of Near-Infrared Emitting Gold Nanoclusters for Biological Applications

Published on: March 22, 2020

Direct Synthesis of EM-Visible Gold Nanoparticles in Cells for Protein Localization Analysis with Well-Preserved Ultrastructure
09:22

Direct Synthesis of EM-Visible Gold Nanoparticles in Cells for Protein Localization Analysis with Well-Preserved Ultrastructure

Published on: April 28, 2023

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay
12:31

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay

Published on: February 28, 2015

Area of Science:

  • Nanomaterials Science
  • Environmental Chemistry
  • Analytical Chemistry

Background:

  • Mercury ions (Hg2+) pose significant environmental and health risks.
  • Accurate and rapid detection of Hg2+ is crucial for pollution monitoring.
  • Existing detection methods can be complex or require specialized equipment.

Purpose of the Study:

  • To develop a simple, selective, and sensitive colorimetric sensor for Hg2+ detection.
  • To utilize gold nanoparticles (AuNPs) modified with thymine acetamidoethanethiol (T-SH) for Hg2+ sensing.
  • To enable naked-eye detection of Hg2+ in aqueous media.

Main Methods:

  • Self-assembly of T-SH on AuNPs to form a modified nanoparticle solution.
  • Induction of T-SH modified AuNPs aggregation via T-Hg-T complex formation in the presence of Hg2+.
  • Colorimetric analysis of the aggregation-induced color change (red to blue-gray).

Main Results:

  • The sensor demonstrated a visual detection limit as low as 0.5 µM for Hg2+.
  • High selectivity was observed, with no response to other common metal ions even at 100-fold higher concentrations.
  • The T-SH/AuNPs system showed high stability and enabled rapid on-site detection.

Conclusions:

  • The developed T-SH/AuNPs sensor offers a straightforward and effective method for Hg2+ detection.
  • Its selectivity and sensitivity make it suitable for environmental monitoring of mercury pollution.
  • The sensor's ease of use and visual output facilitate rapid, on-site analysis.