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Related Concept Videos

Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...

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Updated: Jun 6, 2026

A Polyaniline-based Sensor of Nucleic Acids
07:58

A Polyaniline-based Sensor of Nucleic Acids

Published on: November 1, 2016

Nucleic acid detection using carbon nanoparticles as a fluorescent sensing platform.

Hailong Li1, Yingwei Zhang, Lei Wang

  • 1State Key Lab of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022 Jilin, China.

Chemical Communications (Cambridge, England)
|November 17, 2010
PubMed
Summary

Carbon nanoparticles (CNPs) offer a novel platform for detecting nucleic acids. This method achieves high sensitivity, distinguishing even single-base mismatches in DNA sequences.

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Last Updated: Jun 6, 2026

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Published on: September 27, 2016

Area of Science:

  • Nanotechnology
  • Biochemistry
  • Molecular Biology

Background:

  • Nucleic acid detection is crucial for diagnostics and research.
  • Existing methods may lack sensitivity or specificity.
  • Novel sensing platforms are needed for improved nucleic acid analysis.

Purpose of the Study:

  • To establish carbon nanoparticles (CNPs) as a fluorescent sensing platform for nucleic acid detection.
  • To demonstrate the selectivity of CNPs for detecting single-base mismatches.
  • To validate the mechanism of fluorescence quenching and recovery for sensing.

Main Methods:

  • Adsorption of dye-labeled single-stranded DNA (ssDNA) probes onto CNPs via π-π interactions.
  • Utilizing fluorescence quenching of the dye upon adsorption.
  • Monitoring fluorescence recovery upon hybridization with target double-stranded DNA (dsDNA).

Main Results:

  • Proof of concept for CNPs as a fluorescent sensing platform.
  • Demonstrated selectivity for nucleic acid detection down to single-base mismatch.
  • Successful recovery of dye fluorescence upon formation of dsDNA hybrids.

Conclusions:

  • CNPs provide an effective and selective platform for nucleic acid detection.
  • The π-π interaction-based mechanism enables sensitive detection.
  • This approach holds promise for advanced molecular diagnostics.