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Related Concept Videos

Transgenic Organisms00:53

Transgenic Organisms

Overview
Transgenic Organisms00:53

Transgenic Organisms

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What is Genetic Engineering?00:49

What is Genetic Engineering?

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Transgenic Plants02:50

Transgenic Plants

Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
Gene Therapy00:59

Gene Therapy

Gene therapy is a technique where a gene is inserted into a person’s cells to prevent or treat a serious disease. The added gene may be a healthy version of the gene that is mutated in the patient, or it could be a different gene that inactivates or compensates for the patient’s disease-causing gene. For example, in patients with severe combined immunodeficiency (SCID) due to a mutation in the gene for the enzyme adenosine deaminase, a functioning version of the gene can be inserted. The...
In-vitro Mutagenesis01:16

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.

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Related Experiment Video

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In Vitro Selection of Engineered Transcriptional Repressors for Targeted Epigenetic Silencing
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Transgene design.

Bart van de Sluis1, Jan Willem Voncken

  • 1Department of Pathology & Medical Biology, Medical Biology Section, Molecular Genetics, University Medical Center Groningen, University of Gronignen, Groningen, The Netherlands.

Methods in Molecular Biology (Clifton, N.J.)
|November 17, 2010
PubMed
Summary

Designing transgenic mice requires careful planning. This guide covers essential considerations and methods for creating these powerful gene research models.

Area of Science:

  • Genetics and Genomics
  • Molecular Biology
  • Animal Models

Background:

  • Transgenic mice are essential tools for studying gene function.
  • Understanding gene roles is crucial in biological research.
  • Developing effective transgenic models requires specific expertise.

Purpose of the Study:

  • To provide an overview of designing transgenes.
  • To discuss critical choices for successful transgenic mouse generation.
  • To outline DNA purification methods for microinjection.

Main Methods:

  • Review of design considerations for transgenes.
  • Discussion of strategic choices in model generation.
  • Description of DNA purification techniques.

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Main Results:

  • Key requirements for transgene design are outlined.
  • Important decisions for creating transgenic mice are detailed.
  • Practical methods for DNA purification are presented.

Conclusions:

  • Successful transgenic mouse generation relies on meticulous planning.
  • This chapter offers guidance for researchers in the field.
  • Essential information is provided for creating robust gene function models.