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Updated: Jun 6, 2026

An Efficient Method for Adenovirus Production
10:06

An Efficient Method for Adenovirus Production

Published on: June 10, 2021

Adenovirus-mediated gene transfer.

Ko Willems van Dijk1, Kyriakos E Kypreos, Frits J Fallaux

  • 1Department of Human Genetics, Leiden University Medical Center, Leiden, The Netherlands.

Methods in Molecular Biology (Clifton, N.J.)
|November 17, 2010
PubMed
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This protocol details using the AdEasy system for recombinant adenovirus production, enabling short-term gene expression in mouse liver and cell lines. The comprehensive guide covers gene cloning to in vivo administration over approximately 8 weeks.

Area of Science:

  • Molecular Biology
  • Gene Therapy
  • Virology

Background:

  • Recombinant adenoviruses are valuable tools for transient gene expression.
  • Efficient production systems are crucial for their application.
  • The AdEasy system offers a versatile platform for adenovirus vector development.

Purpose of the Study:

  • To provide a detailed protocol for producing recombinant adenoviruses using the AdEasy system.
  • To guide researchers from gene of interest cloning to in vivo application.
  • To establish a reliable method for short-term gene expression studies.

Main Methods:

  • Utilizing the AdEasy system for recombinant adenovirus construction.
  • Step-by-step cloning of the gene of interest into the viral vector.

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Last Updated: Jun 6, 2026

An Efficient Method for Adenovirus Production
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  • Purification and titration of recombinant adenoviruses.
  • In vivo administration protocols for mouse models.

    • Main Results:

    • Successful production of recombinant adenoviruses is achievable.
    • The AdEasy system facilitates reliable and versatile vector generation.
    • The protocol outlines a complete workflow from cloning to in vivo use.

    Conclusions:

    • The AdEasy system provides a robust method for generating recombinant adenoviruses.
    • This protocol enables efficient short-term gene expression in mouse liver and primary cells.
    • The described process is practical for researchers in gene therapy and molecular biology.