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Related Concept Videos

Subcellular Fractionation01:32

Subcellular Fractionation

The homogenate obtained after cell lysis contains various membrane-bound organelles that can be further separated into pure fractions by subcellular fractionation. These isolates are used to study specific cellular components, analyze localized protein activity, and are even employed in diagnostics. Fractionation is typically achieved using centrifugation methods, the most common being density-gradient and differential centrifugation.
Differential Centrifugation
Differential centrifugation is...
Overview Of Cell Separation And Isolation01:20

Overview Of Cell Separation And Isolation

Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.

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Related Experiment Video

Updated: Jun 6, 2026

Subcellular Fractionation from Fresh and Frozen Gastrointestinal Specimens
10:07

Subcellular Fractionation from Fresh and Frozen Gastrointestinal Specimens

Published on: July 15, 2018

Subcellular fractionation methods and strategies for proteomics.

Yie Hou Lee1, Hwee Tong Tan, Maxey C M Chung

  • 1Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, Singapore.

Proteomics
|November 17, 2010
PubMed
Summary

Subcellular proteomics uses advanced fractionation and bioinformatics to map organelle proteins. Verification methods and integrative analysis are crucial for overcoming challenges in studying cellular structures.

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Related Experiment Videos

Last Updated: Jun 6, 2026

Subcellular Fractionation from Fresh and Frozen Gastrointestinal Specimens
10:07

Subcellular Fractionation from Fresh and Frozen Gastrointestinal Specimens

Published on: July 15, 2018

Cell Fractionation of U937 Cells in the Absence of High-speed Centrifugation
06:53

Cell Fractionation of U937 Cells in the Absence of High-speed Centrifugation

Published on: January 18, 2019

Cell Fractionation of U937 Cells by Isopycnic Density Gradient Purification
10:19

Cell Fractionation of U937 Cells by Isopycnic Density Gradient Purification

Published on: August 12, 2021

Area of Science:

  • Proteomics
  • Cell Biology
  • Bioinformatics

Background:

  • Subcellular fractionation enables organelle and cellular structure protein analysis.
  • Classical and advanced techniques are used for protein profiling.

Purpose of the Study:

  • To review subcellular fractionation techniques for proteomics.
  • To discuss prediction, validation, and challenges in subcellular proteomics.

Main Methods:

  • Classical fractionation (e.g., density gradient centrifugation).
  • Emerging techniques (e.g., fluorescent-assisted organelle sorting).
  • Statistical and bioinformatics tools for protein localization prediction.

Main Results:

  • Review of various fractionation, prediction, and validation methods.
  • Discussion of challenges like organelle dynamics.
  • Emergence of integrative analysis as a powerful tool.

Conclusions:

  • Subcellular proteomics is advancing with new techniques and computational tools.
  • Verification of results is critical for reliable organelle protein profiling.
  • Integrative approaches are key to addressing complexity in subcellular proteomics.