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Updated: Jun 6, 2026

Determination of High-affinity Antibody-antigen Binding Kinetics Using Four Biosensor Platforms
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Determination of High-affinity Antibody-antigen Binding Kinetics Using Four Biosensor Platforms

Published on: April 17, 2017

An integrated bioanalytical platform for supporting high-throughput serum protein binding screening.

Jun Zhang1, Wilson Z Shou, Marianne Vath

  • 1Applied Biotechnology, Bristol-Myers Squibb, 5 Research Parkway, Wallingford, CT 06492, USA. jun.zhang1@bms.com

Rapid Communications in Mass Spectrometry : RCM
|November 17, 2010
PubMed
Summary

An integrated system enhances bioanalysis efficiency for drug discovery by automating mass spectrometry optimization and enabling high-throughput sample analysis. This improves speed and capacity for adsorption, distribution, metabolism, and excretion (ADME) screening.

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Area of Science:

  • Analytical Chemistry
  • Pharmacokinetics
  • Drug Discovery

Background:

  • Liquid chromatography/tandem mass spectrometry (LC/MS/MS) is crucial for quantifying small molecules in drug discovery.
  • Current bioanalysis for adsorption, distribution, metabolism, and excretion (ADME) screening involves time-consuming optimization, high-throughput analysis, and data review.
  • Early drug discovery requires efficient methods for analyzing diverse chemical compounds.

Purpose of the Study:

  • To develop an integrated system for improving the efficiency of ADME bioanalysis.
  • To automate MS/MS optimization and implement high-throughput sample analysis for diverse compounds.
  • To enhance data review and reporting processes in bioanalytical support.

Main Methods:

  • An integrated platform featuring automated MS/MS optimization and staggered parallel LC/MS/MS was developed.
  • A sophisticated software tool was employed for raw data review, calculation, and reporting.
  • A unique sample dilution scheme was introduced to reduce the total number of analytical samples.

Main Results:

  • The integrated platform demonstrated high speed, capacity, and robustness in supporting serum protein binding screening assays.
  • The sample dilution scheme reduced operation time and improved overall throughput.
  • The system achieved acceptable inter-assay consistency for serum protein binding determination across multiple species for over 4000 compounds.

Conclusions:

  • The proposed integrated system significantly enhances the efficiency of ADME bioanalysis in early drug discovery.
  • The automated and high-throughput platform, including a novel dilution scheme, streamlines the process of quantifying small molecules.
  • This approach provides robust and efficient bioanalytical support for large-scale compound screening, such as serum protein binding assays.