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Proteomics

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...

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Related Experiment Video

Updated: Jun 6, 2026

Low Molecular Weight Protein Enrichment on Mesoporous Silica Thin Films for Biomarker Discovery

Low Molecular Weight Protein Enrichment on Mesoporous Silica Thin Films for Biomarker Discovery

Published on: April 17, 2012

High-content affinity-based proteomics: unlocking protein biomarker discovery.

Edward N Brody¹, Larry Gold, Richard M Lawn

¹Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, CO 80309, USA.

Expert Review of Molecular Diagnostics

|November 18, 2010

Summary

High-content proteomics aims to discover protein biomarkers by measuring thousands of proteins. A new aptamer-based technology overcomes limitations of antibody assays, enabling sensitive and specific high-content proteomic analysis for biomarker discovery.

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Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

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Last Updated: Jun 6, 2026

Low Molecular Weight Protein Enrichment on Mesoporous Silica Thin Films for Biomarker Discovery

Low Molecular Weight Protein Enrichment on Mesoporous Silica Thin Films for Biomarker Discovery

Published on: April 17, 2012

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

Area of Science:

Proteomics
Biomarker Discovery
Molecular Diagnostics

Background:

Current molecular diagnostics rely on single protein biomarkers measured by antibody-based assays.
High-content proteomics seeks to measure thousands of human proteins for advanced biomarker discovery.
Existing methods like mass spectrometry and antibody assays face challenges in achieving high-content, high-performance proteomic analysis.

Purpose of the Study:

To review antibody-based protein measurement techniques and their limitations in high-content proteomics.
To introduce a novel affinity-based proteomic technology designed for high-content analysis.
To highlight the potential of this new technology in unlocking protein biomarker discovery.

Main Methods:

Review of antibody-based protein measurement and multiplexed assays.
Analysis of antibody limitations, including cross-reactivity, in high-content proteomics.
Introduction of a new affinity-based proteomic technology utilizing slow off-rate modified aptamers (SOMAmers).

Main Results:

Antibody-based methods have limitations for simultaneous high-content and high-performance proteomic measurements.
A new generation of SOMAmers offers a solution for high-content proteomics.
This aptamer-based technology demonstrates potential for sensitive and specific high-content proteomic analysis.

Conclusions:

Traditional antibody-based methods are insufficient for comprehensive high-content proteomics.
Slow off-rate modified aptamers (SOMAmers) represent a promising advancement in affinity-based proteomic technology.
This novel technology is poised to significantly advance protein biomarker discovery.