Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Surface Appendages of Archaea01:23

Surface Appendages of Archaea

Archaeal surface appendages are highly specialized structures essential for environmental adaptation, encompassing roles in adhesion, biofilm formation, and motility. Among these appendages, pili and archaella stand out for their distinct morphologies and functionalities, enabling archaea to thrive in diverse and often extreme environments.Pili: Adhesion and Biofilm FormationPili are filamentous structures assembled from pilin protein subunits, primarily contributing to adhesion and biofilm...
Mechanism of Filopodia Formation01:39

Mechanism of Filopodia Formation

Filopodia are thin, actin-rich cellular protrusions that play an important role in many fundamental cellular functions. They vary in their occurrence, length, and positioning in different cell types, suggesting their diverse roles.
Their main function is to guide migrating cells during normal tissue morphogenesis or cancer metastasis by recognizing and making initial contacts with the extracellular matrix. However, they can also act as stationary cell anchors or help to establish communication...
Bacterial Protein Maturation01:26

Bacterial Protein Maturation

Bacterial protein maturation is a tightly regulated process that ensures newly synthesized polypeptides achieve correct functional conformations. This maturation involves a series of modifications, folding events, and quality control steps, often assisted by specialized chaperone proteins.N-Terminal ModificationsThe maturation of bacterial polypeptides begins cotranslationally as the polypeptide exits the ribosome. The first amino acid, N-formylmethionine (fMet), is typically modified at the...
Mechanism of Lamellipodia Formation01:31

Mechanism of Lamellipodia Formation

Cells migrating in response to external stimuli form lamellipodia, which are thin membrane protrusions supported by a mesh of linked, branched, or unbranched actin filaments. These actin filaments interact with myosin motor proteins, creating the dynamic actomyosin complex within the cytoskeleton. Contractility, or the ability to generate contractile stress, is inherent to the actomyosin complex. It helps cells detect the stiffness of the surrounding ECM and exert contractile force for...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Mitochondrial OXPHOS integrates immunometabolic cascade for bone regeneration via coupled ATP production and ROS homeostasis.

Redox biologyĀ·2026
Same author

Persistent CD4<sup>+</sup> T cell hyporesponsiveness during recovery from prolonged symptomatic SARS-CoV-2 infection.

Cellular immunologyĀ·2026
Same author

Disruption of Treg Homeostasis in Rheumatoid Arthritis via Ferroptosis-Mediated ETC Collapse and TXK-STAT3/PLCĪ³1 Activation.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)Ā·2026
Same author

Impaired Midkine/Treg Signaling Contributes to Pathogenesis of Preeclampsia.

Hypertension (Dallas, Tex. : 1979)Ā·2026
Same author

Critical role of Psl polysaccharide modulated by AmrZ for biofilm formation and cold adaptation in the spoilage bacterium Pseudomonas fluorescens.

International journal of food microbiologyĀ·2026
Same author

Transforming waste derived phosphate sources into resources for fluoride immobilization by microbially induced phosphate precipitation.

Bioresource technologyĀ·2026

Related Experiment Video

Updated: Jun 6, 2026

In Situ Characterization of Shewanella oneidensis MR1 Biofilms by SALVI and ToF-SIMS
09:56

In Situ Characterization of Shewanella oneidensis MR1 Biofilms by SALVI and ToF-SIMS

Published on: August 18, 2017

Pellicle formation in Shewanella oneidensis.

Yili Liang1, Haichun Gao, Jingrong Chen

  • 1School of Minerals processing and Bioengineering, Central south University, Changsha, PR China.

BMC Microbiology
|November 18, 2010
PubMed
Summary

This study reveals essential requirements for Shewanella oneidensis pellicle formation, highlighting the role of specific cations and the AggA secretion system. Pellicles offer a novel model for studying bacterial communities.

More Related Videos

Production, Crystallization and Structure Determination of C. difficile PPEP-1 via Microseeding and Zinc-SAD
13:34

Production, Crystallization and Structure Determination of C. difficile PPEP-1 via Microseeding and Zinc-SAD

Published on: December 30, 2016

Electrochemical Detection of Deuterium Kinetic Isotope Effect on Extracellular Electron Transport in Shewanella oneidensis MR-1
09:00

Electrochemical Detection of Deuterium Kinetic Isotope Effect on Extracellular Electron Transport in Shewanella oneidensis MR-1

Published on: April 16, 2018

Related Experiment Videos

Last Updated: Jun 6, 2026

In Situ Characterization of Shewanella oneidensis MR1 Biofilms by SALVI and ToF-SIMS
09:56

In Situ Characterization of Shewanella oneidensis MR1 Biofilms by SALVI and ToF-SIMS

Published on: August 18, 2017

Production, Crystallization and Structure Determination of C. difficile PPEP-1 via Microseeding and Zinc-SAD
13:34

Production, Crystallization and Structure Determination of C. difficile PPEP-1 via Microseeding and Zinc-SAD

Published on: December 30, 2016

Electrochemical Detection of Deuterium Kinetic Isotope Effect on Extracellular Electron Transport in Shewanella oneidensis MR-1
09:00

Electrochemical Detection of Deuterium Kinetic Isotope Effect on Extracellular Electron Transport in Shewanella oneidensis MR-1

Published on: April 16, 2018

Area of Science:

  • Microbiology
  • Bacterial Physiology
  • Biofilm Formation

Background:

  • Pellicle formation in Shewanella oneidensis at the air-liquid interface is understudied compared to surface-associated biofilms.
  • Understanding pellicle development is crucial for a comprehensive view of S. oneidensis biofilm strategies.

Purpose of the Study:

  • To elucidate the fundamental requirements for pellicle formation in S. oneidensis.
  • To investigate the underlying mechanisms governing pellicle development at the air-liquid interface.

Main Methods:

  • Investigated the role of oxygen and various cations (Ca(II), Mn(II), Cu(II), Zn(II), Mg(II), Fe(II), Fe(III)) in pellicle formation.
  • Analyzed the contribution of proteins, DNA, and exopolysaccharides to pellicle structure.
  • Conducted mutational analysis of flagella and the AggA type I secretion system.

Main Results:

  • Pellicle formation requires oxygen and specific cations, with Ca(II), Mn(II), Cu(II), and Zn(II) being essential.
  • Proteins are crucial, and exopolysaccharides may be mannose-rich; flagella are not required but influence development rate.
  • The AggA type I secretion system is essential for pellicle formation but not for surface-associated biofilms.

Conclusions:

  • This research systematically characterizes S. oneidensis pellicle formation, enhancing understanding of biofilm development.
  • Pellicles represent a valuable model system for studying bacterial communities and their interactions.