Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Video

Updated: Jun 6, 2026

Characterization of Immune Cells in Human Adipose Tissue by Using Flow Cytometry

Characterization of Immune Cells in Human Adipose Tissue by Using Flow Cytometry

Published on: March 6, 2018

Characterization of human adipose-derived stem cells using flow cytometry.

H Alan Tucker¹, Bruce A Bunnell

¹Center for Stem Cell Research and Regenerative Medicine, Tulane University School of Medicine, New Orleans, LA, USA.

Methods in Molecular Biology (Clifton, N.J.)

|November 18, 2010

Summary

Human adipose-derived stem cells (hASCs) show therapeutic potential via paracrine signaling. A standardized flow cytometry protocol aids in isolating and characterizing hASCs for consistent research comparisons.

Related Concept Videos

Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
In...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

The lipedema common case report form as a research tool: standardizing lipedema data collection.

Frontiers in global women's health·2026

Same author

Evolving Landscape of Glioblastoma Research: Integrating Therapeutic Advances and Diagnostic Frontiers.

Brain sciences·2026

Same author

Editorial: Role of induced pluripotent stem cells (iPSCs) in regenerative medicine, disease modeling and drug discovery.

Frontiers in bioengineering and biotechnology·2025

Same author

Oncogenic effects of ECM remodeling in obesity and breast cancer.

Oncogene·2025

Same author

Adipose Stromal Cell Heterogeneity and Trafficking: Implications for Breast Cancer Metastasis and Obesity.

Frontiers in bioscience (Landmark edition)·2025

Same author

RNA sequencing identifies <i>MAP1A</i> and <i>PTTG1</i> as predictive genes of aging CD264<sup>+</sup> human mesenchymal stem cells at an early passage.

Cytotechnology·2025

Area of Science:

Stem Cell Biology
Immunology
Regenerative Medicine

Background:

Human adipose-derived stem cells (hASCs) possess multipotent differentiation capabilities.
hASCs exert therapeutic effects through paracrine signaling, modulating apoptosis, angiogenesis, and inflammation.
Standardized characterization methods for hASCs are lacking, hindering comparative studies.

Purpose of the Study:

To present a standardized protocol for flow cytometric analysis of hASCs.
To facilitate the isolation and characterization of hASCs.
To enable consistent comparisons of hASCs across different tissue sources.

Main Methods:

Development of a flow cytometry protocol using specific antibody panels.
Analysis of surface epitope patterns on hASCs.

More Related Videos

Manual Isolation of Adipose-derived Stem Cells from Human Lipoaspirates

Manual Isolation of Adipose-derived Stem Cells from Human Lipoaspirates

Published on: September 26, 2013

Related Experiment Videos

Last Updated: Jun 6, 2026

Characterization of Immune Cells in Human Adipose Tissue by Using Flow Cytometry

Characterization of Immune Cells in Human Adipose Tissue by Using Flow Cytometry

Published on: March 6, 2018

Manual Isolation of Adipose-derived Stem Cells from Human Lipoaspirates

Manual Isolation of Adipose-derived Stem Cells from Human Lipoaspirates

Published on: September 26, 2013

Isolation and characterization of hASCs.

Main Results:

A reliable protocol for hASC characterization via flow cytometry was established.
Surface marker analysis aids in hASC identification and isolation.
The protocol allows for direct, uniform comparisons of hASCs from various sources.

Conclusions:

Standardized antibody panels improve hASC isolation and characterization.
This protocol promotes uniformity in hASC research, benefiting the field.
hASCs hold significant therapeutic promise mediated by paracrine factors.