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Updated: Jun 6, 2026

Isolation and Enrichment of Human Adipose-derived Stromal Cells for Enhanced Osteogenesis
Published on: January 12, 2015
Adipose-derived stromal cells: cytokine expression and immune cell contaminants
Pauline Decaunes1, David Estève, Alexia Zakaroff-Girard
1Institut National de la Santé et de la Recherche Médicale (INSERM), Institut de Médecine Moléculaire de Rangueil, Université Toulouse III Paul-Sabatier, Toulouse, France.
Abstract:
The present method describes an immunoselection/depletion approach to isolate the native human adipose tissue-derived progenitor cells that are free from endothelial cells and immune cells by the use of magnetic nanobeads and microbeads coupled to antibodies. Moreover, methods to isolate and to analyse the distinct cell populations that constitute the microenvironment of the human adipose tissue progenitor cells, i.e. mature adipocytes, endothelial cells, and macrophages, are mentioned.

