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Related Concept Videos

Mesenchymal Stem Cells01:19

Mesenchymal Stem Cells

Mesenchymal stem cells (MSCs) are adult stem cells that can differentiate into most connective tissue cell types, except for hematopoietic cells, depending upon the source of MSCs. For example, bone-marrow-derived MSCs (BM-MSCs) can differentiate into osteocytes, hepatocytes, and pancreatic and neuronal cells. MSCs can be isolated from various sources such as bone marrow, placenta, adipose tissue, teeth, and Wharton’s jelly, a gelatinous substance in the umbilical cord. The ease of their access...
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Adult Stem Cells

Stem cells are undifferentiated cells that divide and produce more stem cells or progenitor cells that differentiate into mature, specialized cell types. All the cells in the body are generated from stem cells in the early embryo, but small populations of stem cells are also present in many adult tissues including the bone marrow, brain, skin, and gut. These adult stem cells typically produce the various cell types found in that tissue—to replace cells that are damaged or to continuously renew...

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Related Experiment Video

Updated: Jun 6, 2026

Isolation of Primary Patient-specific Aortic Smooth Muscle Cells and Semiquantitative Real-time Contraction Measurements In Vitro
08:28

Isolation of Primary Patient-specific Aortic Smooth Muscle Cells and Semiquantitative Real-time Contraction Measurements In Vitro

Published on: February 15, 2022

Adipose stem cell differentiation into smooth muscle cells.

Kacey G Marra1, Candace A Brayfield, J Peter Rubin

  • 1Division of Plastic Surgery, Department of Surgery, McGowan Institute for Regenerative Medicine, Department of Bioengineering, University of Pittsburgh, Pittsburgh, PA, USA.

Methods in Molecular Biology (Clifton, N.J.)
|November 18, 2010
PubMed
Summary

Adipose-derived stem cells (ASCs) show potential for smooth muscle cell differentiation. Research explores methods for both in vitro and in vivo applications, particularly for vascular therapies.

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Area of Science:

  • Regenerative Medicine
  • Stem Cell Biology
  • Tissue Engineering

Background:

  • Adipose-derived stem cells (ASCs) are abundant and easily accessible.
  • Limited research exists on differentiating ASCs into functional smooth muscle cells.

Purpose of the Study:

  • To review and describe methodologies for ASC differentiation into smooth muscle cells.
  • To highlight the potential of ASCs in regenerative medicine, especially for vascular therapies.

Main Methods:

  • In vitro differentiation using chemical or mechanical stimulation.
  • Assessment of differentiation via smooth muscle cell marker expression.
  • In vivo studies utilizing animal models for cardiovascular disease and urinary bladder reconstruction.

Main Results:

  • Established methods for in vitro ASC differentiation exist.
  • In vivo studies demonstrate potential applications in cardiovascular disease and urogenital reconstruction.
  • ASCs offer a promising source for autologous smooth muscle cells.

Conclusions:

  • ASCs represent a viable cell source for smooth muscle cell generation.
  • Further investigation into ASC differentiation holds promise for future vascular and reconstructive therapies.