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Related Experiment Video

Updated: Jun 6, 2026

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Cell sheet technology for tissue engineering: the self-assembly approach using adipose-derived stromal cells.

Benoît Labbé1, Guillaume Marceau-Fortier, Julie Fradette

  • 1LOEX (Laboratoire d'Organogénèse Expérimental Organogenesis Laboratory), Centre de Recherche FRSQ du CHA Universitaire de Québec, Québec, QC, Canada.

Methods in Molecular Biology (Clifton, N.J.)
|November 18, 2010
PubMed
Summary

This study details a self-assembly tissue engineering method using human adipose-derived stem cells (ASCs) to create connective and adipose tissues. These engineered tissues are composed entirely of ASCs and their secreted extracellular matrix, offering potential for reconstructive surgery.

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Published on: August 4, 2017

Area of Science:

  • Regenerative Medicine
  • Tissue Engineering
  • Stem Cell Biology

Background:

  • Adipose tissue is a valuable source of adult multipotent stem cells (ASCs).
  • ASCs are crucial in regenerative medicine and reconstructive surgery.
  • Tissue engineering utilizes ASCs and biomaterials for tissue reconstruction.

Purpose of the Study:

  • To describe protocols for generating reconstructed connective and adipose tissues using the self-assembly approach.
  • To detail the use of human adipose-derived stem cells (ASCs) and their secreted extracellular matrix.
  • To provide methods for creating multilayered human tissues for regenerative applications.

Main Methods:

  • Human ASCs are amplified and cell-banked.
  • ASCs are cultured to secrete and assemble extracellular matrix components, forming cell sheets.
  • Individual stromal and adipose sheets are produced and assembled into multilayered tissues.

Main Results:

  • Successful reconstruction of connective and adipose tissues entirely from cultured human ASCs and their endogenous extracellular matrix.
  • Demonstration of the self-assembly approach for generating tissue-engineered substitutes.
  • Production of cell sheets that can be manipulated into thicker, multilayered tissues.

Conclusions:

  • The self-assembly approach enables the creation of functional tissue-engineered constructs from human ASCs.
  • This method facilitates the reconstruction of both connective and adipose tissues.
  • The described protocols offer a pathway for advancing regenerative medicine and reconstructive surgery.