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Updated: Jun 6, 2026

Selective Capture of 5-hydroxymethylcytosine from Genomic DNA
Published on: October 5, 2012
Efficient synthesis of 5-hydroxymethylcytosine containing DNA
Martin Münzel1, Daniel Globisch, Christian Trindler
1Center for Integrated Protein Science, Department of Chemistry, Ludwig-Maximilians-University Munich, Butenandtstr. 5-13, 81377 Munich, Germany.
Abstract:
5-Hydroxymethylcytosine ((5-HOMe)dC) was recently discovered as the sixth base in the mammalian genome. The development of a new phosphoramidite building block is reported, which allows efficient synthesis of (5-HOMe)dC containing DNA. Key steps of the synthesis are a palladium-catalyzed formylation and the simultaneous protection of a hydroxyl and amino group as a cyclic carbamate. DNA synthesis is possible under standard conditions, and deprotection can be carried out with dilute NaOH.

