Introduction to calibration curves in bioanalysis

Analytical standards are used in bioanalytical analysis for the quantitative measurement of large and small molecules in the pharmaceutical industry. This article will introduce the reader to the common, acceptable practices for the use of calibration curves in chromatographic and ligand-binding assays. The discussion presented is a combination of regulatory requirements and the authors' experience in performing bioanalysis. While all possible techniques are not covered, generally acceptable practices are described to serve as a starting point for novice practitioners.