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Calibration Curves: Linear Least Squares

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Introduction to calibration curves in bioanalysis.

Edward Brewer¹, Ernest Bollin

¹Tandem Labs, A LabCorp Company, 115 Silvia Street, West Trenton, NJ 08628, USA. ed.brewer@tandemlabs.com

|November 19, 2010

Summary

This guide explains how to use calibration curves in bioanalytical analysis for accurate quantitative measurement of molecules. It covers common practices for chromatographic and ligand-binding assays, essential for pharmaceutical development.

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Area of Science:

Bioanalytical Chemistry
Pharmaceutical Analysis

Background:

Analytical standards are crucial for quantitative molecular measurement in the pharmaceutical industry.
Bioanalysis relies on accurate quantification of large and small molecules for drug development and quality control.

Purpose of the Study:

To outline common and acceptable practices for using calibration curves in bioanalytical assays.
To provide guidance for novice practitioners in performing bioanalysis using validated methods.

Main Methods:

The article discusses practices applicable to chromatographic assays.
It also covers practices for ligand-binding assays.
The content integrates regulatory requirements with practical experience.

Main Results:

Generally acceptable practices for calibration curve utilization are described.
The focus is on providing a foundational understanding for new bioanalytical scientists.

Conclusions:

Adherence to described practices ensures reliable quantitative measurements in bioanalysis.
This guidance serves as a starting point for implementing robust calibration curve strategies in pharmaceutical settings.