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Related Concept Videos

Testing a Claim about Standard Deviation01:19

Testing a Claim about Standard Deviation

A complete procedure to test a claim about population standard deviation or population variance is explained here.
The hypothesis testing for the claim of population standard deviation (or variance) requires the data and samples to be random and unbiased. The population distribution also must be normal. There is no specific requirement on the sample size as the estimation is based on the chi-square distribution.
As a first step, the hypothesis (null and alternative) concerning the claim about...
Comparing Experimental Results: Student's t-Test01:09

Comparing Experimental Results: Student's t-Test

The t-test is a statistical method used to compare the sample mean with a population mean or compare two means from two data sets. The test statistic is calculated from the standard deviation, mean, and number of measurements in the data set at a selected confidence interval and then compared to a table of critical values at this confidence level. If the test statistic is smaller than the critical value, the null hypothesis is accepted. In this case, we state that the difference between the...
Contaminants and Errors01:16

Contaminants and Errors

Effective sample preparation is crucial for accurate and reliable laboratory analysis. During this process, two significant sources of error can arise: concentration bias from improper sample splitting and contamination caused by methods used to reduce particle size, such as grinding or homogenization. Identifying and minimizing these potential errors is crucial to ensuring the validity of the analysis.
Another key consideration is determining the appropriate number of samples required to...
One-Way ANOVA: Unequal Sample Sizes01:15

One-Way ANOVA: Unequal Sample Sizes

One-way ANOVA can be performed on three or more samples of unequal sizes. However, calculations get complicated when sample sizes are not always the same. So, while performing ANOVA with unequal samples size, the following equation is used:
Bonferroni Test01:10

Bonferroni Test

The Bonferroni test is a statistical test named after Carlo Emilio Bonferroni, an Italian mathematician best known for Bonferroni inequalities. This statistical test is a type of multiple comparison test to determine which means are different than the rest. Bonferroni test can minimize the Type 1 error by reducing the significance level alpha, which otherwise increases with sample pairs.
The means of different samples are first paired in all possible combinations.
The null hypothesis of the...
One-Way ANOVA: Equal Sample Sizes01:15

One-Way ANOVA: Equal Sample Sizes

One-Way ANOVA can be performed on three or more samples with equal or unequal sample sizes. When one-way ANOVA is performed on two datasets with samples of equal sizes, it can be easily observed that the computed F statistic is highly sensitive to the sample mean.
Different sample means can result in different values for the variance estimate: variance between samples. This is because the variance between samples is calculated as the product of the sample size and the variance between the...

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Related Experiment Video

Updated: Jun 6, 2026

Methods of Soil Resampling to Monitor Changes in the Chemical Concentrations of Forest Soils
09:16

Methods of Soil Resampling to Monitor Changes in the Chemical Concentrations of Forest Soils

Published on: November 25, 2016

Incurred sample reanalysis: enhancing the Bland-Altman approach with tolerance intervals.

Fred E Lytle1, Randall K Julian, Amy M Tabert

  • 1Indigo BioSystems, 20 East 91st Street, Indianapolis, IN 46240, USA.

Bioanalysis
|November 19, 2010
PubMed
Summary

This study highlights the importance of incurred sample reanalysis for method performance evaluation. It proposes using Bland-Altman plots with tolerance intervals to assess accuracy and determine sample size for bioanalytical methods.

Related Experiment Videos

Last Updated: Jun 6, 2026

Methods of Soil Resampling to Monitor Changes in the Chemical Concentrations of Forest Soils
09:16

Methods of Soil Resampling to Monitor Changes in the Chemical Concentrations of Forest Soils

Published on: November 25, 2016

Area of Science:

  • Analytical Chemistry
  • Bioanalytical Science
  • Pharmacokinetics

Background:

  • Incurred sample reanalysis (ISR) is crucial for validating bioanalytical methods.
  • Assessing method performance and reliability requires robust statistical tools.
  • Determining appropriate sample sizes for studies impacts data quality.

Purpose of the Study:

  • To introduce a combined approach using Bland-Altman plots and tolerance intervals for ISR.
  • To demonstrate the utility of this method for evaluating bioanalytical method performance.
  • To provide guidance on using this approach for determining minimum sample size.

Main Methods:

  • Visual evaluation of method performance using Bland-Altman plots.
  • Application of tolerance intervals to assess data variability.
  • Calculation of 66.7% tolerance factors and comparison of difference metrics.
  • Analysis of an example dataset to illustrate the methodology.

Main Results:

  • The combination of Bland-Altman plots and tolerance intervals offers a visual assessment of method performance.
  • This approach can aid in determining the minimum sample size required for studies.
  • Probability plots can be used to evaluate error distributions effectively.

Conclusions:

  • The proposed method provides a valuable tool for incurred sample reanalysis.
  • This approach enhances the evaluation of bioanalytical method accuracy and reliability.
  • Further discussion and application of these techniques are encouraged in the scientific community.