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Ion suppression and cannulation locking solutions.

Daniel G Morgan1, Timothy V Olah

  • 1Bioanalytical Research, Pharmaceutical Candidate Optimization, Bristol-Myers Squibb, 5 Research Parkway, Wallingford, CT 06492-7660, USA. daniel.morgan@bms.com

Bioanalysis
|November 19, 2010
PubMed
Summary
This summary is machine-generated.

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Ion suppression in bioanalysis can be caused by cannula locking solutions, not just dosing vehicles. This study identified locking solution components as the source of significant ion suppression in rodent experiments.

Area of Science:

  • Analytical Chemistry
  • Pharmacokinetics
  • Biomedical Research

Background:

  • Ion suppression is a critical challenge in liquid chromatography-tandem mass spectrometry (LC-MS/MS) bioanalysis.
  • It can lead to inaccurate quantitation of analytes in biological matrices.
  • Dosing vehicles are known contributors to ion suppression in in vivo studies.

Purpose of the Study:

  • To investigate an observed phenomenon of ion suppression in rodent experiments.
  • To identify the source of significant ion suppression affecting internal standard signals.
  • To determine if components other than the dosing vehicle contribute to ion suppression.

Main Methods:

  • Liquid chromatography-tandem mass spectrometry (LC-MS/MS) was used for bioanalysis.

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  • Analysis of drug candidates in rodent biological samples.
  • Investigation of potential sources of ion suppression, including cannula locking solutions.

    • Main Results:

    • Significant ion suppression (approximately 60%) was observed for a structural analogue internal standard.
    • The cannula locking solution, used to maintain cannula patency, was identified as the source of this ion suppression.
    • This finding highlights a previously unrecognized source of analytical interference.

    Conclusions:

    • Cannula locking solutions can be a significant source of ion suppression in LC-MS/MS bioanalysis.
    • Researchers should consider locking solutions as a potential cause of ion suppression in rodent studies.
    • Proper characterization of all experimental components is crucial for accurate bioanalysis.