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High-throughput and Comprehensive Drug Surveillance Using Multisegment Injection-Capillary Electrophoresis-Mass Spectrometry
Published on: April 23, 2019
Current technologies and considerations for drug bioanalysis in oral fluid.
E Gallardo1, M Barroso, J A Queiroz
1Centro de Investigação em Ciências da Saúde, Universidade da Beira Interior, 6201-506 Covilhã, Portugal. jqueiroz@ubi.pt
Bioanalysis
|November 19, 2010
Summary
Oral fluid analysis offers a non-invasive alternative to blood or urine testing for drugs. Advances in mass spectrometry have made oral fluid drug testing accurate and reliable for various applications.
Area of Science:
- Analytical Chemistry
- Toxicology
- Forensic Science
Background:
- Oral fluid analysis has evolved significantly over 30 years, becoming popular in pharmacokinetics, drug testing, and forensic toxicology.
- Oral fluid offers a non-invasive, privacy-preserving alternative to traditional biological specimens like blood and urine.
- Drug concentrations in oral fluid are believed to correlate with blood concentrations, making it a valuable alternative specimen.
Purpose of the Study:
- To review the applicability of oral fluid analysis in analytical, clinical, and forensic toxicology.
- To highlight the advantages and disadvantages of oral fluid compared to other biological specimens.
- To discuss technological advancements enabling reliable oral fluid drug testing.
Main Methods:
- Review of published literature on oral fluid drug analysis.
- Focus on analytical techniques, including mass spectrometry (LC-MS, LC-MS/MS).
- Evaluation of sample collection methods and on-site detection devices.
Main Results:
- Significant improvements in sample collection, on-site detection, and analytical methods for oral fluid drug testing.
- Mass spectrometry-based methods (LC-MS/MS) are crucial for achieving the necessary sensitivity with limited sample volumes.
- Oral fluid analysis demonstrates potential as a viable alternative to blood and urine in various toxicological fields.
Conclusions:
- Oral fluid analysis is a rapidly advancing field with growing importance in toxicology.
- Technological progress, particularly in mass spectrometry, has overcome previous limitations in sensitivity and reliability.
- Oral fluid testing presents a promising, non-invasive option for drug monitoring and testing across clinical and forensic settings.
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