Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Sample Preparation for Analysis: Overview

Sample Preparation for Analysis: Overview

Sample preparation is an essential step in the analytical process. It involves preparing a sample so that it can be analyzed accurately. The goal is to extract the analyte, the substance you want to measure, from the sample while removing any components that may interfere with the analysis. Sample preparation techniques vary depending on the physical state of the sample.
Bulk or large solid samples are typically reduced in size using grinding, crushing, or milling techniques to increase the...

High-Performance Liquid Chromatography: Elution Process

High-Performance Liquid Chromatography: Elution Process

In High-Performance Liquid Chromatography (HPLC), the elution process is critical to the separation of analytes and the quality of chromatographic results. Elution describes how compounds move through the column and separate based on their interactions with the mobile and stationary phases. This process determines the resolution, peak shape, and retention times in the chromatogram, which are essential for identifying and quantifying components in complex mixtures. Understanding the elution...

Size-Exclusion Chromatography

Size-Exclusion Chromatography

In size-exclusion chromatography (SEC), also known as molecular-exclusion or gel-permeation chromatography, molecules are separated based on their sizes. This technique is important for separating large molecules such as polymers and biomolecules. The two classes of micron-sized stationary phases encountered in SEC are silica particles and cross-linked polymer resin beads. Both materials are porous, but their pore sizes vary significantly.
Silica particles offer advantages such as rigidity,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

3D-printed pipette-tip device for the efficient extraction of opioids from oral fluids using particulate-coated adhesive tapes as sorptive phases.

Analytica chimica acta·2026

Same author

Uncoupling of nutrient sensing and cell size control by specific defects in ceramide structure.

Biology open·2026

Same author

Centrifugal pipette-tip extraction using pyrolyzed cotton fibers combined with mass spectrometry: High-throughput analysis of methadone and its major metabolite in human urine.

Mikrochimica acta·2026

Same author

Sulfonated polystyrene nanospheres from waste sources for the extraction of sulfonamide antibiotics from complex matrices.

Mikrochimica acta·2026

Same author

Clinical Outcomes after Implantation of the Toric Tecnis Eyhance Intraocular Lens: a Retrospective Analysis.

Klinische Monatsblatter fur Augenheilkunde·2026

Same author

Stainless Steel-Synthesized gold Nanoparticles: Insights into Blood-Brain barrier permeability and microglial interaction.

International journal of pharmaceutics·2025

Search research articles

Related Experiment Video

Updated: Jun 6, 2026

A Simple Fractionated Extraction Method for the Comprehensive Analysis of Metabolites, Lipids, and Proteins from a Single Sample

A Simple Fractionated Extraction Method for the Comprehensive Analysis of Metabolites, Lipids, and Proteins from a Single Sample

Published on: June 1, 2017

Liquid-phase microextraction in bioanalytical sample preparation.

Rafael Lucena¹, Marta Cruz-Vera, Soledad Cárdenas

¹Department of Analytical Chemistry, Marie Curie building (Annex), Campus de Rabanales, University of Cordoba, E-14071, Cordoba, Spain. qa1meobj@uco.es

|November 19, 2010

Summary

This summary is machine-generated.

Liquid-phase microextraction (LPME) offers an advanced alternative to traditional liquid-liquid extraction for bioanalytical sample preparation. This technique provides high preconcentration and sample clean-up using minimal sample volumes.

More Related Videos

Extraction and Analysis of Microbial Phospholipid Fatty Acids in Soils

Extraction and Analysis of Microbial Phospholipid Fatty Acids in Soils

Published on: August 26, 2016

Multi-step Preparation Technique to Recover Multiple Metabolite Compound Classes for In-depth and Informative Metabolomic Analysis

Multi-step Preparation Technique to Recover Multiple Metabolite Compound Classes for In-depth and Informative Metabolomic Analysis

Published on: July 11, 2014

Related Experiment Videos

Last Updated: Jun 6, 2026

A Simple Fractionated Extraction Method for the Comprehensive Analysis of Metabolites, Lipids, and Proteins from a Single Sample

A Simple Fractionated Extraction Method for the Comprehensive Analysis of Metabolites, Lipids, and Proteins from a Single Sample

Published on: June 1, 2017

Extraction and Analysis of Microbial Phospholipid Fatty Acids in Soils

Extraction and Analysis of Microbial Phospholipid Fatty Acids in Soils

Published on: August 26, 2016

Multi-step Preparation Technique to Recover Multiple Metabolite Compound Classes for In-depth and Informative Metabolomic Analysis

Multi-step Preparation Technique to Recover Multiple Metabolite Compound Classes for In-depth and Informative Metabolomic Analysis

Published on: July 11, 2014

Area of Science:

Analytical Chemistry
Biochemistry
Separation Science

Background:

Liquid-phase microextraction (LPME) emerged as an improvement over classical liquid-liquid extraction in the late 1990s.
LPME addresses limitations of older extraction methods, offering advantages like reduced sample volume and enhanced purity.
Its development has been driven by widespread adoption in analytical sciences.

Purpose of the Study:

To review the primary techniques within liquid-phase microextraction (LPME).
To highlight the bioanalytical applications of LPME.
To discuss emerging trends, novel extraction modes, and suitable solvents for LPME.

Main Methods:

Focus on single-drop microextraction (SDME) as a key LPME technique.
Review supported hollow-fiber liquid-phase microextraction (HF-LPME).
Analyze trends in extraction modes and solvent selection for LPME.

Main Results:

LPME offers significant advantages for bioanalytical sample preparation, including high preconcentration factors.
The technique excels in achieving excellent sample clean-up.
Single-drop microextraction and supported hollow-fiber LPME are prominent methods within the field.

Conclusions:

LPME is a highly effective technique for bioanalytical sample preparation due to its efficiency and low sample requirements.
Continued development in extraction modes and solvents promises further advancements in LPME.
LPME techniques are crucial for modern analytical and bioanalytical applications.