Liquid-phase microextraction in bioanalytical sample preparation
Related Experiment Video
Updated: Jun 6, 2026

A Simple Fractionated Extraction Method for the Comprehensive Analysis of Metabolites, Lipids, and Proteins from a Single Sample
Published on: June 1, 2017
Rafael Lucena1, Marta Cruz-Vera, Soledad Cárdenas
1Department of Analytical Chemistry, Marie Curie building (Annex), Campus de Rabanales, University of Cordoba, E-14071, Cordoba, Spain. qa1meobj@uco.es
Abstract:
Liquid-phase microextraction (LPME) emerged in the mid-to-late 1990s, facing up to the main shortcomings of the classical liquid-liquid extraction. Since its origin, this new technique has been in continuous development driven by its successful and widespread use in the analytical sciences. Its inherent properties, such as low sample volume requirement, high preconcentration factors achieved and excellent sample clean-up, make LPME a very useful technique for bioanalytical sample preparation. This review focuses on the main LPME-related techniques, predominantly single-drop microextraction and supported hollow-fiber LPME, paying particular attention to the bioanalytical applications. A general view of the essential trends, including the description of promising extraction modes and solvents, is also highlighted.
Related Concept Videos
Sample Preparation for Analysis: Overview
Bulk or large solid samples are typically reduced in size using grinding, crushing, or milling techniques to increase the...
High-Performance Liquid Chromatography: Elution Process
Size-Exclusion Chromatography
Silica particles offer advantages such as rigidity,...

