Automated sample preparation for regulated bioanalysis: an integrated multiple assay extraction platform using robotic liquid handling
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Published on: December 18, 2020
Joseph A Tweed1, Zhenhua Gu, Hui Xu
1Pharmacokinetics, Dynamics & Metabolism, Pfizer Global Research and Development, Pfizer Inc., Groton, CT 06340, USA. joseph.tweed@pfizer.com
Background:
A novel approach for regulated bioanalytical sample preparation has been developed to combine multiple types of extraction techniques into one integrated and automated sample-preparation suite that pairs a graphical user interface with the Hamilton Microlab(®) STAR robotic liquid handler.
Results:
The multi-assay sample-preparation suite is composed of three bioanalytical extraction techniques: protein precipitation, solid-phase extraction and liquid-liquid extraction. Validation data provided highly reproducible and robust results for each respective automated extraction technique.
Conclusion:
The user-friendly graphical user interface and modular method design provide a flexible and versatile approach for routine bioanalytical sample-preparation and is the first fully integrated multiple assay sample-preparation suite for regulated bioanalysis.
