Bioanalytical solutions to acetonitrile shortages

Area of Science:

• Bioanalytical Chemistry
• Analytical Chemistry

Background:

• The 2008-2009 acetonitrile shortage significantly impacted bioanalytical scientists.
• Acetonitrile is crucial for sample preparation and chromatographic analysis.

Purpose of the Study:

• To explore strategies for overcoming acetonitrile scarcity in bioanalytical workflows.
• To identify alternative solvents, reduction techniques, and recycling methods for acetonitrile.

Main Methods:

• Investigated methanol as a substitute for acetonitrile in protein precipitation and chromatography.
• Evaluated methods to reduce solvent consumption, including smaller column dimensions and particle sizes.
• Examined emerging technologies that minimize or eliminate the need for chromatography.
• Assessed the efficacy of acetonitrile recycling systems.

Main Results:

• Methanol proved to be a viable substitute for acetonitrile in protein precipitation and chromatography, especially for phospholipid interference.
• Reduced column dimensions and particle sizes significantly decreased solvent consumption and run times.
• New technologies like desorption ESI and laser diode thermal desorption offered substantial solvent and time savings.
• Acetonitrile recycling systems effectively purified HPLC waste for reuse.

Conclusions:

• The acetonitrile shortage drove the adoption of alternative solvents, optimized chromatographic methods, and novel analytical technologies.
• These strategies not only addressed the shortage but also led to more efficient and cost-effective bioanalytical processes.