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Related Concept Videos

Flow Cytometry01:23

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
In...
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...

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Updated: Jun 6, 2026

Use of Image Cytometry for Quantification of Pathogenic Fungi in Association with Host Cells
07:58

Use of Image Cytometry for Quantification of Pathogenic Fungi in Association with Host Cells

Published on: June 19, 2013

Ongoing development of image cytometers.

Jason S Kim1, Daesung Hur, Jeoung Ku Hwang

  • 1Nanoentek Inc., Ace Highend Tower, 12th floor, 235-2 Kuro-3 Dong, Kuro-Ku, Seoul, Korea. jason.kim@digital-bio.com

Bioanalysis
|November 19, 2010
PubMed
Summary
This summary is machine-generated.

Image cytometry offers affordable cellular analysis for labs and clinics. New fluorescence capabilities expand its applications, despite current limitations compared to advanced systems.

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Area of Science:

  • Biotechnology
  • Cell Biology
  • Medical Technology

Background:

  • Image cytometry provides quantitative cellular analysis using imaging techniques.
  • It utilizes cost-effective components like LED excitation and CCD detectors.
  • Unlike flow cytometry, it analyzes stationary samples with lower light intensity and longer exposure.

Purpose of the Study:

  • To review the technical aspects and applications of image cytometers.
  • To discuss recent advancements and available commercial devices in the field.
  • To highlight the growing potential of image cytometry in various settings.

Main Methods:

  • Image acquisition from slides or microfabricated chips.
  • Data processing using cell recognition software for identification, counting, and analysis.
  • Incorporation of fluorescence detection in newer generation devices.

Main Results:

  • Inexpensive image cytometers are suitable for small laboratories and clinics.
  • Current systems may not match the data quality of high-end flow cytometers or microscopes.
  • The addition of fluorescence detection broadens the applicability of image cytometry.

Conclusions:

  • Image cytometry presents a cost-effective solution for cellular analysis.
  • Technological progress, especially fluorescence, enhances its utility.
  • Further development is needed to match the performance of advanced cytometers.