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A Protocol for Analyzing Hepatitis C Virus Replication
13:04

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Published on: June 26, 2014

Distinguishing acute from chronic hepatitis C virus (HCV) infection based on antibody reactivities to specific HCV

Aufra C Araujo1, Irina V Astrakhantseva, Howard A Fields

  • 1Division of Viral Hepatitis, Centers for Disease Control and Prevention, 1600 Clifton Rd. NE, MS A-33, Atlanta, GA 30333, USA. AAraujo@cdc.gov

Journal of Clinical Microbiology
|November 19, 2010
PubMed

Currently available serological assays for detection of antibodies to hepatitis C virus (HCV) cannot reliably discriminate acute from chronic HCV infection. We developed a multiplexed, flow-cytometric microsphere immunoassay to measure anti-HCV-IgG reactivities to the core, NS3, NS4, and NS5 HCV recombinant proteins and applied it to 99 serum samples from 24 anti-HCV seroconverters and 141 anti-HCV-IgG and HCV RNA-positive plasma specimens from chronically infected people. Differences in the geometric means or means of signal/cutoff ratios between the two sample sets were statistically significant for all the antigens tested. A multivariate logistic regression model correctly classified the samples in two groups, with a cross-validation accuracy of 90.8% for the acute group and 97.2% for the chronic group. The immunoassay described has the potential to distinguish acute from chronic HCV infection.

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