Multiple-locus variable-number tandem repeat analysis of Neisseria gonorrhoeae
Raymond Heymans1, Leo M Schouls, Han G J van der Heide
1GGD, Public Health Laboratory, Cluster of Infectious Diseases, Amsterdam Health Service, Nieuwe Achtergracht 100, 1018 WT Amsterdam, Netherlands. rheymans@ggd.amsterdam.nl
Journal of Clinical Microbiology
|November 19, 2010
Summary
Molecular typing of Neisseria gonorrhoeae using multiple-locus variable-number tandem repeat analysis (MLVA) accurately identified distinct transmission networks. This method revealed transmission clusters among men who have sex with men (MSM) and heterosexual populations in Amsterdam.
Area of Science:
- Microbiology
- Epidemiology
- Molecular Biology
Background:
- Rising Neisseria gonorrhoeae prevalence in the Netherlands necessitates advanced molecular typing.
- Understanding transmission networks in high-risk populations is crucial for public health interventions.
Purpose of the Study:
- To develop and evaluate a multiple-locus variable-number tandem repeat analysis (MLVA) for Neisseria gonorrhoeae.
- To elucidate molecular epidemiology and transmission networks in Amsterdam's high-risk groups.
Main Methods:
- Developed an MLVA assay using 5 variable-number tandem repeat (VNTR) loci.
- Amplified VNTR loci via multiplex PCR and analyzed on an automated sequencer.
- Assessed VNTR locus stability and performed hierarchical cluster analysis on MLVA profiles.
Main Results:
- MLVA typing demonstrated high stability across anatomical sites and sexual partners (87-88% shared MLVA type).
- Hierarchical clustering of 716 isolates identified 14 distinct clusters, with 7 predominantly from men who have sex with men (MSM) and 5 from heterosexuals.
- Clusters showed variations in ethnic background and coinfections, indicating distinct transmission patterns.
Conclusions:
- MLVA is a reliable tool for accurately identifying genetically related Neisseria gonorrhoeae strains.
- The study successfully revealed distinct transmission networks within MSM and heterosexual populations in Amsterdam.
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