Related Experiment Video

Updated: Jun 6, 2026

Proton Transfer and Protein Conformation Dynamics in Photosensitive Proteins by Time-resolved Step-scan Fourier-transform Infrared Spectroscopy
10:03

Proton Transfer and Protein Conformation Dynamics in Photosensitive Proteins by Time-resolved Step-scan Fourier-transform Infrared Spectroscopy

Published on: June 27, 2014

Fast time-resolved Fourier-transform spectroscopy for the study of transient chemical reactions

C A Carere, W S Neil, J J Sloan

    Applied Optics
    |November 19, 2010
    PubMed

    More Related Videos

    Ultrafast Time-resolved Near-IR Stimulated Raman Measurements of Functional π-conjugate Systems09:57

    Ultrafast Time-resolved Near-IR Stimulated Raman Measurements of Functional π-conjugate Systems

    Published on: February 10, 2020

    Time-resolved Photophysical Characterization of Triplet-harvesting Organic Compounds at an Oxygen-free Environment Using an iCCD Camera06:08

    Time-resolved Photophysical Characterization of Triplet-harvesting Organic Compounds at an Oxygen-free Environment Using an iCCD Camera

    Published on: December 27, 2018

    Related Concept Videos

    Fast Reactions01:27

    Fast Reactions

    Fast reactions occurring in times shorter than the time needed to mix reactants pose a unique challenge for investigation. In a liquid-phase continuous-flow system, reactants A and B are swiftly pushed into the mixing chamber, where mixing occurs within 1 ms. The reaction mixture then flows through an observation tube, and one measures light absorption to determine species concentrations at various points of the tube. This method is most appropriate when relatively large volumes of reactants...
    Total Internal Reflection Fluorescence Microscopy01:05

    Total Internal Reflection Fluorescence Microscopy

    Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
    Continuous -time Fourier Transform01:11

    Continuous -time Fourier Transform

    The Fourier series is instrumental in representing periodic functions, offering a powerful method to decompose such functions into a sum of sinusoids. This technique, however, necessitates modification when applied to nonperiodic functions. Consider a pulse-train waveform consisting of a series of rectangular pulses. When these pulses have a finite period, they can be accurately represented by a Fourier series. Yet, as the period approaches infinity, resulting in a single, isolated pulse, the...
    Protein Dynamics in Living Cells01:19

    Protein Dynamics in Living Cells

    Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
    Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
    Measuring Reaction Rates03:09

    Measuring Reaction Rates

    Polarimetry finds application in chemical kinetics to measure the concentration and reaction kinetics of optically active substances during a chemical reaction. Optically active substances have the capability of rotating the plane of polarization of linearly polarized light passing through them—a feature called optical rotation. Optical activity is attributed to the molecular structure of substances. Normal monochromatic light is unpolarized and possesses oscillations of the electrical field in...
    Tandem Mass Spectrometry01:21

    Tandem Mass Spectrometry

    Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...