Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.
Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Comparative study of BCR-ABL1 quantification: Xpert assay, a feasible solution to standardization concerns.

Annals of hematology·2012
Same author

Ultrasound monitoring of the setting of calcium-based bone cements.

Journal of materials science. Materials in medicine·2012
Same author

Epidemiology and prognostic determinants of bacteraemic biliary tract infection.

The Journal of antimicrobial chemotherapy·2012
Same author

Src tyrosine kinase inhibits apoptosis through the Erk1/2- dependent degradation of the death accelerator Bik.

Cell death and differentiation·2012
Same author

Evidence-based guideline update: intraoperative spinal monitoring with somatosensory and transcranial electrical motor evoked potentials: report of the Therapeutics and Technology Assessment Subcommittee of the American Academy of Neurology and the American Clinical Neurophysiology Society.

Neurology·2012
Same author

Magnifying lens for 800 MeV proton radiography.

The Review of scientific instruments·2011
Same journal

Multifunctional reconfigurable terahertz metasurface based on vanadium dioxide phase transition: achieving broadband absorption and efficient polarization conversion.

Applied optics·2026
Same journal

High-Q-factor electromagnetically induced transparency utilizing quasi-bound states in the continuum in an all-dielectric terahertz metasurface.

Applied optics·2026
Same journal

Automated stitching interferometry for high-precision metrology of X-ray mirrors.

Applied optics·2026
Same journal

Experimental demonstration of an approach to designing a metal-dielectric DBR resonant cavity structure.

Applied optics·2026
Same journal

High-precision wavefront reconstruction from a single-shot interferogram using a physics-driven hybrid feature calibration network.

Applied optics·2026
Same journal

Ultra-high-Q Fano resonance based on coupled topological corner states in Kagome photonic crystals.

Applied optics·2026
See all related articles

Related Experiment Video

Updated: Jun 6, 2026

Live Cell Imaging of F-actin Dynamics via Fluorescent Speckle Microscopy (FSM)
19:16

Live Cell Imaging of F-actin Dynamics via Fluorescent Speckle Microscopy (FSM)

Published on: August 5, 2009

Resolution-enhanced detection of moving objects embedded within scattering media using time-gated speckle methods.

P Naulleau, D Dilworth, E Leith

    Applied Optics
    |November 19, 2010
    PubMed
    Summary
    This summary is machine-generated.

    The holographic first-arriving-light method improves resolution for detecting moving objects in scattering media. This technique offers better results than standard speckle differencing alone.

    More Related Videos

    Time Multiplexing Super Resolving Technique for Imaging from a Moving Platform
    06:25

    Time Multiplexing Super Resolving Technique for Imaging from a Moving Platform

    Published on: February 12, 2014

    Spectral Reflectometric Microscopy on Myelinated Axons In Situ
    09:13

    Spectral Reflectometric Microscopy on Myelinated Axons In Situ

    Published on: July 2, 2018

    Related Experiment Videos

    Last Updated: Jun 6, 2026

    Live Cell Imaging of F-actin Dynamics via Fluorescent Speckle Microscopy (FSM)
    19:16

    Live Cell Imaging of F-actin Dynamics via Fluorescent Speckle Microscopy (FSM)

    Published on: August 5, 2009

    Time Multiplexing Super Resolving Technique for Imaging from a Moving Platform
    06:25

    Time Multiplexing Super Resolving Technique for Imaging from a Moving Platform

    Published on: February 12, 2014

    Spectral Reflectometric Microscopy on Myelinated Axons In Situ
    09:13

    Spectral Reflectometric Microscopy on Myelinated Axons In Situ

    Published on: July 2, 2018

    Area of Science:

    • Optics and Photonics
    • Biomedical Imaging
    • Laser Physics

    Background:

    • Scattering media obscure object detection.
    • Conventional imaging methods lack resolution for embedded objects.
    • Speckle differencing is a common technique for object detection.

    Purpose of the Study:

    • To enhance the resolution of moving object detection in scattering media.
    • To evaluate the effectiveness of combining holographic first-arriving-light with speckle differencing.
    • To compare the proposed method against standard speckle differencing.

    Main Methods:

    • Holographic first-arriving-light (FAL) technique.
    • Speckle differencing method.
    • Combination of FAL and speckle differencing for enhanced detection.

    Main Results:

    • The combined method achieved resolution-enhanced detection of moving objects.
    • Significant resolution improvements were observed compared to standard speckle differencing.
    • The holographic FAL technique proved crucial for the enhanced resolution.

    Conclusions:

    • The combination of holographic first-arriving-light and speckle differencing is effective for resolution-enhanced object detection in scattering media.
    • Holographic FAL offers superior resolution compared to standard speckle differencing.
    • This technique has potential applications in various fields requiring imaging through scattering media.