[Enhancing hyperthermic cytotoxicity in Walker-256 by intraarterial injection of warmed saline]

The tumor growth of Walker-256 implanted sc. on dorsum side of hindpaw of Wistar rat were suppressed by warmed (43 degrees C) water immersion. Antitumor effects of hyperthermia were increased by injection (a.i.) of saline mixed with noradrenaline (5 mcg). Although tumors in advanced stage group (D-8) are larger in size and more tumor vascularity than in early stage group (D-4), hyperthermic cytotoxicity were observed in D-8 but not observed in D-4. The hyperthermic energy injured the tumor vessels, which failed to flow the blood to the tumor cells and resulted in sever cytotoxic damage of Walker-256. Therefore, cytotoxic damage could be enhanced by injecting warmed water (43 degrees C) into tumor vessels after chemotherapy (MMC; 0.5 mg/kg) in D-8. Metastatic liver cancer was treated with thermochemotherapy. 5% of glucose warmed (43 degrees C) mixed with 0.1 mg of noradrenaline and heparin was administered into hepatic artery after chemotherapy (2 to 6 mg of MMC a.i.).