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Related Concept Videos

Structure of Cadherins01:25

Structure of Cadherins

The cadherins were one of the first cell adhesion molecules discovered; the term “cadherins”   is based on their calcium-dependent adhering properties. The first cadherins discovered on the epithelial, neuronal, and placental cells were named E-cadherin, P-cadherin, and N-cadherin, respectively. These classical cadherins share sequence and structural similarities. Other cadherins, including those involved in cell signaling, are grouped into non-classical cadherins. This diversity of cadherins...

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Bead Aggregation Assays for the Characterization of Putative Cell Adhesion Molecules
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Concentrated diffusing colloidal probes of Ca2+-dependent cadherin interactions.

W Neil Everett1, Daniel J Beltran-Villegas, Michael A Bevan

  • 1Chemical Engineering, Texas A&M University, College Station, Texas 77843, United States.

Langmuir : the ACS Journal of Surfaces and Colloids
|November 20, 2010
PubMed
Summary
This summary is machine-generated.

Calcium (Ca2+) triggers N-cadherin attraction, mediating bilayer fusion at >100 μM. Simulations reveal N-cadherin bond formation and attraction begin at significantly lower Ca2+ concentrations (0.1 μM).

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Area of Science:

  • Biophysics
  • Materials Science
  • Cell Adhesion

Background:

  • N-cadherin mediates cell-cell adhesion, crucial for tissue development and integrity.
  • Understanding cadherin interactions at the molecular level is vital for developmental biology and disease research.

Purpose of the Study:

  • To investigate calcium (Ca2+)-dependent interactions between N-cadherin fragments.
  • To characterize N-cadherin-mediated attraction and bilayer fusion in a quasi-2D system.
  • To determine the precise Ca2+ concentration threshold for N-cadherin bond formation.

Main Methods:

  • Video microscopy measurements of N-cadherin fragments on supported lipid bilayers.
  • Inverse simulation analyses using Monte Carlo and Brownian Dynamics.
  • Characterization of bilayer formation, fluidity, and fragment attachment.

Main Results:

  • Nonspecific repulsion observed between cadherin fragments without Ca2+.
  • Irreversible bilayer fusion mediated by N-cadherin attraction at Ca2+ concentrations >100 μM.
  • Simulations indicated measurable N-cadherin attraction starting at 0.1 μM Ca2+.

Conclusions:

  • N-cadherin-mediated attraction and bilayer fusion are Ca2+-dependent processes.
  • The effective Ca2+ concentration for N-cadherin attraction is lower than previously reported.
  • This study provides new insights into the biophysical mechanisms of cadherin-mediated adhesion.