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Epizootic rabbit enteropathy: comparison of PCR-based RAPD fingerprints from virulent and non-virulent samples

N Huybens1, J Houeix, D Licois

  • 1Department of Clinical Science, University of Liège, 20 Boulevard de Colonster, 4000 Liège, Belgium. nathalie.huybens@ulg.ac.be

Veterinary Journal (London, England : 1997)
|November 20, 2010
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Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...