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Related Concept Videos

Lymphoid Cells and Tissues01:18

Lymphoid Cells and Tissues

Lymphoid cells and tissues are integral to the immune system, which is crucial in maintaining our body's defense against harmful pathogens. They form the building blocks of lymphoid organs, which include the spleen, thymus, and lymph nodes.
Lymphoid cells consist of various types of immune system cells. These include B and T lymphocytes, which are responsible for producing antibodies and killing infected cells, respectively. Dendritic cells act as messengers between the innate and adaptive...

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Isolation and Quantification of Epstein-Barr Virus from the P3HR1 Cell Line
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Lymphomas differ in their dependence on Epstein-Barr virus.

David T Vereide1, Bill Sugden

  • 1McArdle Laboratory for Cancer Research, University of Wisconsin, Madison, WI 53706, USA.

Blood
|November 20, 2010
PubMed
Summary

Epstein-Barr virus (EBV) plays a varied role in lymphomas. Some tumors need EBV to prevent cell death, while others rely on it for growth and survival, showing a graded viral dependence.

Area of Science:

  • Oncology
  • Virology
  • Molecular Biology

Background:

  • Epstein-Barr virus (EBV) is linked to various lymphomas, including Burkitt lymphoma (BL) and posttransplant lymphoproliferative disorder (PTLD).
  • The precise role of EBV in driving lymphomagenesis and maintaining tumor cell survival is complex and varies across lymphoma subtypes.

Purpose of the Study:

  • To investigate the conditional dependence of different lymphoma cells on Epstein-Barr virus (EBV) by excising the viral genome.
  • To elucidate the specific functions EBV serves in maintaining the viability and proliferation of tumor cells in BL and PTLD.

Main Methods:

  • Conditional eviction of the extrachromosomal EBV genome from cultured lymphoma cells in vitro.
  • Assessment of apoptosis induction, cell cycle progression, and proliferation in EBV-dependent and EBV-independent tumor cells.

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Separation of Immune Cell Subpopulations in Peripheral Blood Samples from Children with Infectious Mononucleosis
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Main Results:

  • Canonical Burkitt lymphoma (BL) cells showed minimal dependence on EBV, with some requiring it to prevent apoptosis.
  • Wp-restricted BL cells demonstrated a strong dependence on EBV to inhibit apoptosis (involving Bim regulation) and promote proliferation.
  • Posttransplant lymphoproliferative disorder (PTLD) cells required EBV to inhibit apoptosis and drive cell cycle progression (G1/G0 exit).

Conclusions:

  • Lymphoma tumor cells exhibit a graded dependence on EBV, correlating with the number of viral genes expressed.
  • EBV contributes differentially to tumor cell survival and proliferation, highlighting its multifaceted oncogenic role in various lymphomas.