Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Cell Specific Gene Expression01:58

Cell Specific Gene Expression

Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
Cell Specific Gene Expression01:58

Cell Specific Gene Expression

Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
Ribosome Profiling02:24

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...
What is Gene Expression?01:36

What is Gene Expression?

A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is comprised  of nucleotides and proteins are comprised of amino acids, a mediator is required to convert the information encoded in DNA into proteins. This mediator is the messenger RNA (mRNA). mRNA copies the blueprint from DNA by a process called transcription. In eukaryotes, transcription occurs in the nucleus by complementary base-pairing with the DNA template. The mRNA is then processed and...
What is Gene Expression?01:42

What is Gene Expression?

Overview
Gene expression is the process in which DNA directs the synthesis of functional products, that is, proteins. Cells can regulate gene expression at various stages. It allows organisms to generate different cell types and enables cells to adapt to internal and external factors.
Genetic Information Flows from DNA to RNA to Protein
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is made up of nucleotides and proteins consist of amino...
Regulation of Expression at Multiple Steps01:23

Regulation of Expression at Multiple Steps

The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the addition of a...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The contributions of ammonia oxidizing bacteria and archaea to nitrification-dependent N<sub>2</sub>O emission in alkaline and neutral purple soils.

Scientific reports·2022
Same author

MEK1-dependent MondoA phosphorylation regulates glucose uptake in response to ketone bodies in colorectal cancer cells.

Cancer science·2022
Same author

Interface engineering with porous graphene as deposition regulator of stable Zn metal anode for long-life Zn-ion capacitor.

Journal of colloid and interface science·2022
Same author

Fragment-Based Dynamic Combinatorial Chemistry for Identification of Selective α-Glucosidase Inhibitors.

ACS medicinal chemistry letters·2022
Same author

Insulin-binding protein-5 down-regulates the balance of Th17/Treg.

Frontiers in immunology·2022
Same author

Hsa_circ_0094606 promotes malignant progression of prostate cancer by inducing M2 polarization of macrophages through PRMT1-mediated arginine methylation of ILF3.

Carcinogenesis·2022

Related Experiment Video

Updated: Jun 6, 2026

Real-time Analysis of Transcription Factor Binding, Transcription, Translation, and Turnover to Display Global Events During Cellular Activation
12:54

Real-time Analysis of Transcription Factor Binding, Transcription, Translation, and Turnover to Display Global Events During Cellular Activation

Published on: March 7, 2018

Systematic analysis of gene expression level with tissue-specificity, function and protein subcellular localization

Qiang Li1, Xianghua Liu, Quanyuan He

  • 1State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, 220 Handan Road, Shanghai, 200433, People's Republic of China. lq054@hotmail.com

Molecular Biology Reports
|November 20, 2010
PubMed
Summary

Gene expression levels in mammals correlate with tissue specificity, protein function, and cellular location. Highly expressed genes are widely distributed and conserved across evolution.

More Related Videos

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations
09:34

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

Published on: October 25, 2018

Related Experiment Videos

Last Updated: Jun 6, 2026

Real-time Analysis of Transcription Factor Binding, Transcription, Translation, and Turnover to Display Global Events During Cellular Activation
12:54

Real-time Analysis of Transcription Factor Binding, Transcription, Translation, and Turnover to Display Global Events During Cellular Activation

Published on: March 7, 2018

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations
09:34

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

Published on: October 25, 2018

Area of Science:

  • Genomics
  • Molecular Biology
  • Bioinformatics

Background:

  • Mammalian gene expression studies indicate shorter genes and slower protein evolution for highly expressed genes.
  • The comprehensive relationship between gene expression and features like tissue specificity, function, and subcellular localization in mammals remains underexplored.

Purpose of the Study:

  • To investigate the global correlations between gene expression levels and tissue-specificity, protein function, and subcellular localization in human genes.
  • To analyze 8,570 genes across 46 human tissues to understand these relationships.

Main Methods:

  • Analysis of gene expression data from 46 human tissues.
  • Comparison of gene expression levels with gene length, tissue specificity, protein function (e.g., DNA-binding, zinc-finger proteins), and subcellular localization (e.g., nuclear, cytoplasmic, mitochondrial).

Main Results:

  • Widely expressed genes exhibit higher mean expression levels than tissue-specific genes.
  • Genes encoding zinc-finger proteins show low expression levels, comparable to DNA-binding proteins.
  • Nuclear and Golgi apparatus proteins have lower expression than mitochondrial, endoplasmic reticulum, and membrane proteins; cytoplasmic and extracellular proteins have the highest expression.
  • Variations in gene expression activity and transcript encoding were observed across different tissues.

Conclusions:

  • Gene expression levels are significantly correlated with tissue specificity, protein function, and subcellular localization in humans.
  • These expression patterns are conserved during evolution, suggesting fundamental biological importance.