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An inducible expression vector for both fission and budding yeast.

D Picard¹, M Schena, K R Yamamoto

¹Department of Biochemistry and Biophysics, University of California, San Francisco 94143-0448.

|February 14, 1990

Summary

We created a new inducible gene expression system for fission yeast and budding yeast. This system uses glucocorticoid hormones to control gene activity, offering a 20-70 fold induction range.

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Area of Science:

Molecular Biology
Yeast Genetics

Background:

Controlling gene expression is crucial for biological research.
Existing methods in yeast may lack precise inducibility or broad applicability.

Purpose of the Study:

To develop a novel vector system for inducible gene expression in both Schizosaccharomyces pombe and Saccharomyces cerevisiae.
To enable precise control of gene expression using a widely available external signal.

Main Methods:

Construction of an autonomously replicating expression vector.
Incorporation of multiple glucocorticoid response elements for hormone-mediated induction.
Utilizing the mammalian glucocorticoid receptor to activate gene expression.

Main Results:

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Achieved 20-70 fold induction of gene expression in response to glucocorticoid hormones.

Demonstrated successful application in both fission yeast and budding yeast.

Verified that glucocorticoids act as gratuitous inducers with minimal off-target effects.

Conclusions:

The developed vector system provides a robust and tunable method for inducible gene expression in yeast.
This system facilitates the study of gene function and regulation in two distinct yeast models.
Offers a valuable tool for researchers in molecular biology and synthetic biology.