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Induction of new ADAM related proteins from treated human Chang-liver cells
1Department of Biochemistry and Molecular Biology, Xinxiang Medical University, 453003 Xinxiang, China.
Abstract:
Chang-liver cells is a cell line generated from human liver tissue, which is often used in scientific research. ADAMs are a family of proteins that consist of multi-domains, possess multi-functions and play a central role in normal or abnormal physiological conditions, such as regeneration and tumorigenesis. To investigate the expression and functional alteration of the ADAMs or ADAM related proteins in Chang-liver cells, this cell line was treated with heat stress, modified Hanks solution containing ATP or other buffers. Our results showed that the treatment with Hanks solution containing ATP induces Chang-liver cells to express new ADAM related proteins. To analyze these new ADAM related proteins, a cDNA expression library was constructed for the treated Chang-liver cells. A series of positive clones were obtained through immunoscreening with an ADAMs common antibody. A new ADAM related protein possessing alkaline protease activity was confirmed in these clones.
Insights
Researchers identified novel ADAM-related proteins in Chang-liver cells using ATP-modified Hanks solution. These proteins exhibit alkaline protease activity, offering new insights into liver cell biology and potential therapeutic targets.
Area of Science:
- Biochemistry
- Cell Biology
- Proteomics
Background:
- Chang-liver cells are a human liver cell line utilized in scientific research.
- ADAMs (a disintegrin and metalloproteinase domain) proteins are crucial in physiological processes like regeneration and tumorigenesis.
Purpose of the Study:
- To investigate the expression and functional changes of ADAMs or ADAM-related proteins in Chang-liver cells.
- To identify novel ADAM-related proteins induced by specific treatments.
Main Methods:
- Chang-liver cells were treated with heat stress and modified Hanks solution containing ATP.
- A cDNA expression library was constructed from treated cells.
- Immunoscreening with an ADAMs common antibody was performed.
Main Results:
- Treatment with Hanks solution containing ATP induced the expression of new ADAM-related proteins in Chang-liver cells.
- A novel ADAM-related protein with alkaline protease activity was identified from the constructed library.
Conclusions:
- ATP-modified Hanks solution can induce the expression of novel ADAM-related proteins in Chang-liver cells.
- The identified protein possesses alkaline protease activity, suggesting a role in cellular functions.
